UHPLC法同时测定金银花中6种有效成分的含量  

Simultaneous Determination of Six Compounds in Lonicera japonica Thunb by UHPLC

作　　者：韩永成[1] 刘伟[1] 陈宁[1] 崔永霞[1] 黄丽杰[1] 

机构地区：[1]河南中医学院分析测试中心,郑州450008

出　　处：《天然产物研究与开发》2015年第1期89-93,共5页Natural Product Research and Development

基　　金：河南省教育厅自然科学研究计划项目(2010A360016)

摘　　要：本实验建立了UHPLC同时测定不同产地金银花中绿原酸、芦丁、木犀草苷、异绿原酸A、异绿原酸B、异绿原酸C含量的分析方法。采用Agilent 1290超高效液相色谱系统,Agilent ZORBAX RH C18色谱柱（50 mm×2.1 mm,1.8μm）,流动相为乙腈-0.2%磷酸水,以0.2 m L/min的流速进行梯度洗脱,柱温30℃,检测波长350nm。结果表明在该色谱条件下,金银花的6种有效成分在8 min内可达到基线分离。方法的加样回收率为96.88%~99.16%,相对标准偏差为0.23%~1.06%。UHPLC法分析速度快,重复性好,结果准确,可用于金银花中绿原酸、芦丁、木犀草苷、异绿原酸A、异绿原酸B、异绿原酸C的含量测定。To establish an UHPLC method for simultaneous determination of six compounds,namely chlorogenic acid,rutin,luteoloside,3,5-O-dicaffeoylquinic acid,3,4-O-dicaffeoylquinic acid and 4,5-O-dicaffeoylquinic acid in Lonicera japonica Thunb from different areas. The chromatographic separation was performed on an Agilent ZORBAX RH C18（50 mm × 2. 1 mm,1. 8 μm） column with gradient elution. The flow rate was 0. 2 m L/min. The column temperature was 30 oC. The detection wavelength was 350 nm. The recoveries of rutin,luteoloside and four kinds of organic acids were96. 88%-99. 16%,and the relative standard deviations were 0. 23%-1. 06%. The developed UHPLC method was reproducible,accurate and efficient. It can be used for the simultaneous detection and determination of chlorogenic acid,rutin,luteoloside,3,5-O-dicaffeoylquinic acid,3,4-O-dicaffeoylquinic acid,4,5-O-dicaffeoylquinic acid in L. japonica.

关 键 词：金银花 UHPLC 绿原酸 芦丁 木犀草苷 异绿原酸A 异绿原酸B 异绿原酸C 

分 类 号：R917[医药卫生—药物分析学]