老龙七水提液对兔离体肠平滑肌收缩的影响  

Effects of Water Extract of Lobaria retigera on Isolated Rabbit Intestine Smooth Muscle Contraction

作　　者：李志东[1] 王慧[2] 张海娟[2] 陈现伟[2] 胡开良[2] 刘旭海[3] 冯尚彩[2] 

机构地区：[1]广州中医药大学研究生院,广州510006 [2]临沂大学山东省鲁南中药材资源开发工程技术研究中心,山东临沂276000 [3]临沂市人民医院,山东临沂276003

出　　处：《中国药房》2015年第4期463-466,共4页China Pharmacy

基　　金：山东省科技发展计划资助项目(No.2012GNC11027);临沂大学博士科研启动经费资助项目

摘　　要：目的：研究老龙七水提液对兔离体肠平滑肌收缩的影响。方法：以0.25、0.50、0.75、1.00、1.25 g（生药）/L老龙七水提液、0.003 125 g/L乙酰胆碱（先加入）＋1.25 g（生药）/L老龙七水提液（后加入）、0.003 125 g/L阿托品（先加入）＋1.25 g（生药）/L老龙七水提液（后加入）作用于兔离体肠平滑肌,记录兔离体肠平滑肌收缩频率、振幅和张力。结果：0.75~1.25 g（生药）/L老龙七水提液可明显增强兔离体肠平滑肌自发性收缩的振幅和张力（P〈0.01）,且呈浓度依赖性,但对频率无影响;1.25 g（生药）/L老龙七水提液可明显增强乙酰胆碱诱导的兔离体肠平滑肌收缩的振幅和张力（P〈0.05）,并且可明显增强阿托品诱导的兔离体肠平滑肌舒张后的振幅和张力（P〈0.05）。结论：老龙七水提液可增强乙酰胆碱诱导的兔离体肠平滑肌的收缩,可对抗阿托品诱导的兔离体肠平滑肌舒张。OBJECTIVE： To study the effects of water extract of Lobaria retigera on isolated rabbit intestine smooth muscle contraction. METHODS： The isolated intestinal smooth muscle of rabbits were treated with 0.25, 0.50, 0.75, 1.00 and 1.25 g （me- dicinal materials）/L water extract ofL. retigera, 0.003 125 g/L acetylcholine （adding first）＋1.25 g （medicinal material）/L water ex- tract ofL. retigera （adding secondly）, 0.003 125 g/L atropine （adding first）＋1.25 g （medicinal material）/L water extract ofL. retig- era （adding secondly）. The frequency, amplitude and contractive tension of isolated intestine smooth muscle contractions were re- corded. RESULTS： 0.75-1.25 g （medicinal materials）/L water extract of L. retigera could enhance the amplitude and contractive ten- sion of isolated intestine smooth muscle spontaneous contractions significantly （P〈0.01） in concentration-dependent manner, but had no effect on its frequency. 1.25 g （medicinal materials）/L water extract of L. retigera could enhance the amplitude and contrac- tive tension of acetylcholine-induced intestine smooth muscle contractions significantly and also increased contraction rate （P〈 0.05） ; 1.25 g （medicinal materials）/L water extract of L. retigera could enhance the amplitude and contractive tension of atro- pine-induced intestine smooth muscle relaxation significantly and also increased contraction rate （P〈0.05）. CONCLUSIONS： The water extract of L. retigera can coordinate acetylcholine-induced isolated intestine smooth muscle contractions of rabbits, and resist atropine-induced intestinal smooth muscle relaxation.

关 键 词：老龙七 水提液 兔 肠平滑肌 收缩作用 

分 类 号：R285[医药卫生—中药学]