大孔吸附树脂AB-8对芒果苷吸附及扩散动力学的影响  

Influence of the Adsorption and Diffusion Kinetics of Macroporous Absorbent Resin AB-8 on Mangiferin

作　　者：邓妍芳[1] 索绪斌[1] 黄旖旎[1] 宋文霞[1] 

机构地区：[1]广东药学院中药学院,广州510006

出　　处：《中国药房》2015年第4期480-484,共5页China Pharmacy

摘　　要：目的:研究大孔吸附树脂AB-8对芒果苷的吸附及扩散动力学影响。方法:将1g干树脂置于200 ml芒果苷溶液(0.02mg/ml)中振荡过夜,测定大孔吸附树脂AB-8吸附量以计算静态吸附率与静态解吸率,从而确定等温线模型。将1g大孔吸附树脂AB-8置于0.04、0.025、0.012 5、0.006 25、0.003 125 mg/ml芒果苷溶液中,分别在303、313、323 K下恒温振荡器中振荡24h,测定溶液中芒果苷含量以计算平衡吸附量,从而确定热力学参数。将1g大孔吸附树脂AB-8分别置于0.04、0.025 mg/ml芒果苷溶液中,于303 K恒温条件下桨法吸附,测定大孔吸附树脂AB-8吸附量并绘制芒果苷吸附动力学曲线,从而确定吸附动力学模型。采用反相高效液相色谱法测定芒果苷含量。色谱柱为Kromasil C18,流动相为甲醇-0.1%磷酸(35∶65,V/V),流速为1 ml/min,检测波长为259 nm,柱温为室温,进样量为20μl。结果:芒果苷质量浓度在0.001 25~0.02 mg/ml范围内与峰面积积分值呈良好线性关系(r=0.999 8),精密度试验RSD均小于0.75%,回收率为98.77%~100.67%,稳定性试验RSD为1.17%。大孔吸附树脂AB-8对芒果苷的静态吸附率与解吸率分别为82.73%、87.10%。大孔吸附树脂AB-8对芒果苷的吸附特征以Freundlich方程描述更准确,其属于多分子层吸附,以物理吸附为主。大孔吸附树脂AB-8对芒果苷的整个吸附过程趋向于准二级动力学模型,芒果苷在大孔吸附树脂AB-8的吸附是自发放热物理过程。结论:芒果苷在大孔吸附树脂AB-8上的吸附率和解吸率较高,室温下能自发进行,增加大孔吸附树脂AB-8孔径可提高其扩散速率。大孔吸附树脂AB-8是一种分离纯化芒果苷的理想树脂。OBJECTIVE： To study the influence of absorption and diffusion kinetics of macroporous absorbent resin AB-8 on mangiferin. METHODS： The static absorption rate and resolution rate were calculated by testing macraporous resin AB-8 absorp- tion, with dry resin 1 g into mangiferin solution （0.02 mg/ml） shaken overnight, and then determine Freundlich model. Dry resin 1 g were added into mangiferin solution （0.04,0.025,0.012 5,0.006 25,0.003 125 mg/ml） shaking for 24 h in the temperature oscilla- tor with 303,313,323 K respectively to test the content of mangiferin solution to determine the equilibrium absorption capacity and thermodynamic parameters. With the slurry solution, dry resin 1 g were put into mangiferin solution 0.04,0.025 mg/ml respectively with 303 K to test AB-8 absorption quantity macraporous absorbent resin AB-8 absorption, draw the mangiferin absorption kinetic curve and determine the absorption kinetic model. The content of mangiferin was tested by RP-HPLC, with Kromasil C^8 column, mobile phase of methanol-0.1% phosphoric acid （35：65, V/V）, at flow rate of 1 ml/min, with detection wavelength of 259 nm, room temperature and injection volume of 20 μl. RESULTS： The concentration of mangiferin within 0.001 25-0.02 mg/ml showed a good linear relationship （r=0.999 8） with peak area values. RSD values of precision test were less than 0.75%, recoveries were 98.77%-100.67%, RSD of stability test was 1.17%. The static absorption rate and resolution rate of macraporous absorbent resin AB-8 on mangiferin were 82.73%, 87.10%. Freundlich equation can describe the adsorption characteristics of macraporous absor- bent resin AB-8 on mangiferin, and it belongs to multi-molecular layer adsorption with physical adsorption. The whole adsorption process of macraporous absorbent resin AB-8 on mangiferin tended to quasi-second-order kinetic model that the absorption was a spontaneous physical adsorption process with heat releasing. CONCLUSIONS： The absorption rate and resolution rate of macrapor-

关 键 词：芒果苷 大孔吸附树脂AB-8 热力学 动力学 

分 类 号：R284.2[医药卫生—中药学] O643.12[医药卫生—中医学]