正交试验法优选荆芥橙皮苷的提取工艺  

Optimization of Extraction Technology of Hesperidin from Schizonepeta tenuifolia by Orthogonal Test

作　　者：杨静[1] 杨晓君[1] 

机构地区：[1]新疆农业大学食品科学与药学学院

出　　处：《中国药房》2015年第4期532-534,共3页China Pharmacy

摘　　要：目的:优选荆芥药材中橙皮苷的提取工艺。方法:以橙皮苷含量为考察指标,采用高效液相色谱法,通过单因素试验考察不同超声提取时间、料液比、提取次数、乙醇体积分数对橙皮苷含量的影响;通过正交试验L(934)确定荆芥橙皮苷提取的最优提取工艺,并进行验证试验。结果:优选的提取工艺为50%乙醇溶液超声提取1次,提取时间为30 min,料液比为1∶30(g∶ml)。3次验证试验结果显示橙皮苷的含量分别为0.27%、0.28%、0.28%。结论:该提取工艺稳定、可行,适合荆芥橙皮苷的提取。OBJECTIVE： To optimize the extraction technology of hesperidin from Schizonepeta tenuifolia. METHODS： With the index of hesperidin content, HPLC was used to study the effects of different ultrasonic extraction time, solid-liquid ratio, ex- traction times, and ethanol volume fraction on the content of hesperidin by single factor test. L9 （34） orthogonal test was employed to optimize the extraction technology of hesperidin from S. tenuifolia with following verification test. RESULTS： The optimum ex- traction conditions were ultrasonic extraction for 1 time with 50% ethanol, 30 min extraction time and 1 ： 30 （g ： ml） ratio of sol- id-liquid. The contents of hesperidin were 0.27%, 0.28% and 0.28% in the three verification tests. CONCLUSIONS： The extrac- tion technology is stable, feasible and suitable for hesperidin extraction.

关 键 词：荆芥 单因素试验 正交试验 橙皮苷 高效液相色谱法 提取工艺 

分 类 号：R284.2[医药卫生—中药学]