Effects of Traditional Chinese Herbal Medicine on the Structure of Duodenal Mucosa of Chickens under Heat Stress  

中草药对热应激鸡十二指肠黏膜结构的影响（英文）

作　　者：高桂生[1] 陈娟[1] 刘欢[1] 邢超[1] 史秋梅[1] 沈萍[1] 高光平[1] 张艳英[1] 

机构地区：[1]河北科技师范学院/河北省预防兽医学重点实验室,河北秦皇岛066004

出　　处：《Agricultural Science & Technology》2015年第1期103-106,146,共5页农业科学与技术（英文版）

基　　金：Supported by Agricultural Science and Technology Achievement Transformation Project of the Ministry of Science and Technology(2012GB2A200044);Project of Science and Technology Department of Hebei Province(13826615D)~~

摘　　要：[Objective] This study aimed to investigate the effects of traditional Chinese herbal medicine on the structure of duodenal mucosa of chickens under heat stress. [Method] One hundred and twenty 88-day-old Isa Brown chickens were randomly divided into six groups, including three control groups （normal temperature control group, high temperature control group, high temperature Vc control group） and three high-temperature administration groups （high-dose administration group, moderate-dose administration group, low-dose administration group）. Chickens in normal temperature control group were reared at 14-25 ℃, and those in other five groups were reared at 28-39 ℃. The experiment lasted 10 d. Five chickens in each group were euthanized at 1, 4, 8 and 10 d post-treatment, respectively. The duodenal mucosa was collected and prepared into tissue slices with the conventional method for hematoxylin-eosin （HE） staining. Mucosal thickness, villus length and crypt depth of duodenal mucosa were measured. [Result] Under heat stress, duodenal mucosal thickness and villus length were both significantly lower than those in normal temperature control group, and the duodenal villi were scarce and thin. However, under high temperature conditions, various indicators in high-dose and moderate-dose administration groups were higher than those in high temperature control group and high temperature Vc control group, which exhibited extremely significant differences at 8 and 10 d post-treatment, especially. Moreover, various indicators in high-dose administration group were significantly higher than those in moderate-dose and low-dose administration groups. The traditional Chinese herbal medicine prescription exhibited higher protective effects on duodenal mucosa of chickens under heat stress compared with high temperature Vc control group. [Conclusion] Under heat stress, traditional Chinese herbal medicine reduced effectively the duodenal mucosa damage in chickens.伊莎褐88日龄雏鸡120只,随机分为6组,3个对照组（常温组、高温组、高温Vc组）和3个中草药高温组（高剂量组、中剂量组、低剂量组）。常温组在14~25℃环境下饲养,其余5组在28~39℃环境下饲养.试验全程共10天,在试验的第1、4、8、10天每组各剖杀5只并取其十二指肠。经常规方法制成组织切片后H.E染色,测定各十二指肠的黏膜厚度、绒毛长度、隐窝深度。结果显示：热应激状态下,十二指肠黏膜厚度和绒毛长度均显著低于常温组,且绒毛稀疏。但是高温条件下,中草药高剂量组、中剂量组各项指标均高于高温组和高温Vc组,特别是在试验的第8天和第10天差异极显著,并且中草药高剂量组各指标显著高于中草药中低剂量组。该处方对热应激鸡十二指肠粘膜的保护作用强于高温Vc组。在热应激条件下中草药有效减弱了十二指肠粘膜的组织损伤。

关 键 词：Traditional Chinese herbal medicine Heat stress Mucosal thickness Villus length Villus length to crypt depth ratio 

分 类 号：S853.7[农业科学—临床兽医学]