Adsorption Characteristics of Resins to Antibacterial Substances in the Fermentation Broth of Micromonospora carbonacea  

树脂对炭样小单孢菌发酵液中抗菌物质吸附特性的研究（英文）

作　　者：刘晶鑫[1] 黄运红[1] 戴冷 龙中儿[1] 

机构地区：[1]江西师范大学生命科学学院,江西省亚热带植物资源保护与利用重点实验室,江西南昌330022

出　　处：《Agricultural Science & Technology》2015年第1期116-119,共4页农业科学与技术（英文版）

基　　金：Supported by the National Natural Science Foundation of China(31360018,31160029);the Natural Science Foundation of Jiangxi Province(20132BAB204007,20122BAB204008);Science and Technology Planning Project from Educational Commission of Jiangxi Province(GJJ12181);Special Fund for Agro-scientific Research in the Public Interest(201203072)~~

摘　　要：[Objective] In this study, the feasibility of resins to extract antibacterial substances in ferrfientation broth of Micromonospora carbonacea JXNU-1 was investigated. Various parameters including the type of resin, the time of adsorption and the initial pH on the extraction efficiency were investigated and optimized. [Method] The antibacterial activity of antibiotics was determined by agar diffusion method. [Result] The 717 anion resin was selected as the optimal, and its adsorption capacity reached 1 221 U/g within 2.5 h at the acidic range of pH （pH 5）. The Freundlich model was fit for the adsorption model of the 717 anion resin for antibiotics. [Conclusion] The results laid the foundation for further separating and purifying antibiotics in fermentation broth of Micromonospora carbonacea JXNU-1.[目的]研究树脂从炭样小单孢菌发酵液中分离纯化抗生素的可行性,并探讨树脂吸附动力学及吸附等温线。[方法]采用琼脂糖扩散方法检测发酵液中抗生素的生物活性。[结果]717阴离子树脂能够吸附炭样小单孢菌发酵液中抗生素。在pH5.0,吸附时间为2.5 h的条件下,717阴离子树脂的吸附量为1 221 U/g,吸附等温线符合Freundlich模型。[结论]该研究将为分离纯化炭样小单孢菌发酵液中抗生素奠定基础。

关 键 词：Adsorption characteristics Micromonospora carbonacea RESIN ANTIBIOTICS 

分 类 号：Q93[生物学—微生物学]