ELISA Detection of Antibody against Canine Distemper Virus in Fox  

狐狸犬瘟热抗体的ELISA检测（英文）

作　　者：张艳英[1] 卢会鹏 王晓姗[1] 郭杨柳 吴楠[1] 李艳云[1] 史秋梅[1] 高超[1] 

机构地区：[1]河北科技师范学院,河北省预防兽医学重点实验室,河北昌黎066600

出　　处：《Agricultural Science & Technology》2015年第1期120-124,共5页农业科学与技术（英文版）

基　　金：Supported by Hebei Science and Technology Support Program(10960408D;14826613D);Research and Development Plan of Shijiazhuang Municipal Science and Technology Bureau(09150923A);Hebei Province Department of Education Fund(Z2007212);Qinhuangdao Academy of Agricultural Sciences Fund(2014-04)~~

摘　　要：Canine distemper is one of the most important common viral epidemics in farmed foxes. In this study, the whole blood samples of foxes that had been immunized with canine distemper vaccine were collected from a farming area in Changli County. The antibody against canine distemper virus in the 85 prepared serum samples were detected with a double-antibody sandwich ELtSA method. The results showed among the 85 samples from the 4 farms, 81 samples were antibody-positive, and only 4 samples had no antibody against canine distemper. The overall antibody level was higher in that farming area. It is indicated canine distemper vaccination and vaccination program have gotten attention from most of the local farmers, and the immune effect of canine distemper vaccine is better.从昌黎县某养殖小区中,采集经犬瘟热疫苗免疫接种后的狐狸全血,制备血清后,应用双夹心ELISA法对狐狸免疫接种犬瘟热病毒后85份样品进行抗体检测。结果显示,该养殖小区4个养殖户的85份样品有81例呈阳性,4例呈阴性,抗体水平较高,说明该地区犬瘟热的免疫和免疫程序得到大部分养殖户的重视,免疫保护效果较好。

关 键 词：Canine distemper in fox ANTIBODY ELISA 

分 类 号：S858.92[农业科学—临床兽医学]