巴山榧树及近缘种的psbA-trnH序列分析  

PsbA-trnH Sequence Analysis of Torreya fargesii and its Closely Related Species

作　　者：周先容[1] 尚进[1] 陈发波[1] 宋晓宏[1] 江波[1] 

机构地区：[1]长江师范学院,重庆涪陵408100

出　　处：《西部林业科学》2015年第1期16-21,共6页Journal of West China Forestry Science

基　　金：国家自然科学基金项目(31470568);重庆市自然科学基金项目(cstc2012jjA00006);教育部"春晖计划"项目(Z2011144);长江师范学院科研创新团队建设计划资助项目(2014XJTD06)

摘　　要：采用PCR产物直接测序法对巴山榧树12个地理种群的叶绿体psb A-trn H序列进行了测定,并从Gen Bank搜索并下载巴山榧树近缘种的psb A-trn H序列,运用Clustal X和MEGA 4.1软件分析和构建系统发育树。结果表明,巴山榧树12个地理种群间的遗传分化程度较低;巴山榧树与云南榧、日本榧树的亲缘关系较近,而与长叶榧、榧树、加州榧和佛罗里达榧的亲缘关系较远。研究结果为进一步探讨巴山榧树分子谱系地理学及榧树属的分子系统发育提供了理论依据。Chloroplast psbA-trnH intergenic spacer sequences of 12 geographical populations of Torreya fargesii were sequenced by using PCR products sequencing method.psbA-trnH sequences of its closely related species were retrieved from GenBank.Clustal X and MEGA 4.1 were used to analyze the psbA-trnH sequences among these geo-graphical populations of T.fargesii and its closely related species, and to construct its phylogenetic tree.The re-sults showed that genetic differentiation among 12 geographical populations of T.fargesii was low.T.fargesii had close corelation with T.fargesii var.yunnanensis and T.nucifera, but had minor corelation with T.jackii, T.grandis, T.californica and T.taxifolia.The results of this research could provide theoretical foundation for fur-ther investigation on molecular phylogeography of T.fargesii and phylogeny of Torreya.

关 键 词：巴山榧树 地理种群 近缘种 psbA-trnH序列 系统发育树 

分 类 号：S791.53[农业科学—林木遗传育种]