磷酸化核转录因子-κB在骨癌痛大鼠脊髓背角的表达变化  

Expression of phospho-nuclear factor-κB in the spinal dorsal horn in a rat model of tibiai bone cancer pain

作　　者：孔双明 申文[2] 胡学铭[1] 刘彦楠[1] 张婷[1] 曹守斌[1] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室,江苏省麻醉与镇痛应用技术重点实验室,221004 [2]徐州医学院附属医院疼痛科

出　　处：《国际麻醉学与复苏杂志》2015年第1期31-34,共4页International Journal of Anesthesiology and Resuscitation

摘　　要：目的观察骨癌痛大鼠脊髓水平磷酸化核转录因子-κB（phospho-nuclear factor—κB，p-NF-κB）的表达位置及表达变化。方法雌性未交配Sprague-Dawley（SD）大鼠48只，体重160g-200g，采用随机数字表法将其随机分为两组：假手术组（S组）、骨癌痛（bonecan cerpain，BCP）组（BP组），每组24只。选用von Frey纤维丝检测各组大鼠造模前及造模后3、7、14、21d机械缩足阈值（mechanical withdrawal threshold，MWT），评价疼痛行为学变化。采用免疫荧光法检测S组和BP组术前1d及术后7、14、21d大鼠脊髓L4-6节段背角Ⅰ、Ⅱ板层p-NF-κB阳性细胞数的变化。免疫荧光双标法检测脊髓L4-6节段背角Ⅰ、Ⅱ板层p-NF-κB与神经元标志物神经元特异核蛋白（neuronal nuclei，NeuN）、小胶质细胞标志物离子钙接头蛋白分子-1（ionized calcium bindingad aptormolecule-1，Iba-1）、星形胶质细胞标志物胶质纤维酸性蛋白（glia fibrillary acidic protein，GFAP）共表达情况。结果各组肿瘤细胞接种前MWT比较差异无统计学意义（P〉0．05）；与S组比较，BP组肿瘤细胞接种后第7天MWT[（3．57±1．29）g]、第14天MWT[（1．66±0．39）g]、第21天MWT[（1．40±0．16）g]降低（P〈0．05）；S组各时间点MWT差异无统计学意义（P〉0．05）。与S组比较，BP组大鼠术后7d脊髓L4-6节段背角Ⅰ、Ⅱ板层p-NF-κB表达水平升高，随时间延长逐渐升高（P〈0．05）。p-NF-κB几乎都与GFAP共表达，而很少与NeuN、Iba-1共表达。结论BCP大鼠脊髓背角水平星形胶质细胞内p-NF-κB参与介导BCP大鼠机械痛敏。Objective To investigate the expression of spinal phospho-nuclear factor-κB （p-NF-κB） in a rat model of tibial bone cancer pain. Methods Forty-eight female Sprague-Dawley （SD） rats weighting 160 g-200 g were randomly divided into two groups： sham operation group （group S） and bone cancer pain（BCP） group（group BP）. Before operation and at 3,7, 14,21 d after operation, changes of pain behavior of rats in each group were measured using von Frey filaments. The rats were sacrificed on one day before and 7,14,21 d after operation and L4-6 segments of the spinal cords were removed for measurement of the expression of p-NF-κB protein by fluorescent immunohistochemistry. Double immunostaining for p-NF-r,B with cell-specific markers： neuronal nuclei （NeuN）, gtia fibrillary acidic protein （GFAP） and ionized calcium binding adaptor molecule-1 （lba-1） was performed to identify the cell types that expressed p-NF-κB. Results Behavioral assays reveals that the mechanical withdrawal threshold （MWT） was significantly decreased at 7 d [（3.57±1.29） g], 14 d [（1.66±0.39） g], 21 d [（1.40±0.16） g] after operation （P〈0.05） and decreased gradually with the progress of the disease in the group BP. Compared with that in group S, the expression of p-NF-κB was significantly increased at 7 d after operation （P〈0.05） and increased gradually afterwards in the group BP. p-NF-κB was primarily coexpressed with GFAP, but little with NeuN and Iba-1 of group BP rats. Conclusions The expression of p-NF-κB in spinal astroeytes was involved in the development of tibial bone cancer pain.

关 键 词：骨癌痛 磷酸化核转录因子-κB 脊髓背角 星形胶质细胞 

分 类 号：R614[医药卫生—麻醉学] R738.1[医药卫生—外科学]