葡萄籽原花青素对白色念珠菌抑菌作用的体外研究  

The inhibitory effect of grape seed proanthocyanidins on Candida albicans in vitro

作　　者：董波[1] 刁天娇 李益玲[2] 李冬梅[3] 付月明[2] 

机构地区：[1]佳木斯大学附属口腔医院,黑龙江佳木斯154003 [2]佳木斯大学口腔医学院,黑龙江佳木斯154007 [3]佳木斯大学附属口腔医院牙体牙髓病一科,黑龙江佳木斯154003

出　　处：《中国微生态学杂志》2015年第1期49-51,共3页Chinese Journal of Microecology

摘　　要：目的研究葡萄籽原花青素对白色念珠菌的抑菌作用,通过观察其对生物膜活性的影响,探讨其在口腔微生态中变化的意义。方法采用MTT法确定葡萄籽原花青素体外对白色念珠菌的抑菌作用,激光共聚焦显微镜观察不同浓度药物作用后的效果,并进行红绿荧光染色定量分析。结果与阴性对照组相比,最小抑菌浓度为32 mg/m L,在所实验的浓度范围内随着药物浓度的增加抑菌性逐渐增大,CLSM观察,葡萄籽原花青素作用于白色念珠菌生物膜后可使生物膜内活菌比例下降,生物膜活性降低。结论葡萄籽原花青素对白色念珠菌起到了明显的抑制作用。Objective To study the inhibitory effect of grape seed proanthocyanidins on Candida albicans by observing its impact on biofilm formation,and explore its role in the changes of oral microflora. Methods MTT assay was used to determine the minimum inhibitory concentrations of grape seed proanthocyanidins against Candida albicans in vitro. Confocal laser scanning microscope（ CLSM） was used to observe the effects of different concentrations of the drug. Red and green fluorescence staining was performed for quantitative analysis. Results The minimum inhibitory concentration was 32 mg / m L. The inhibitory effect increased along with the concentration of the drug within the scope of the concentration being tested. CLSM showed that grape seed proanthocyanidins reduced the proportion of viable cells in Candida albicans biofilm and inhibited the activity of the biofilm. Conclusion Grape seed proanthocyanidins can significantly inhibit Candida albican in vitro.

关 键 词：葡萄籽原花青素 白色念珠菌 生物膜 

分 类 号：R379.4[医药卫生—病原生物学]