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机构地区:[1]阜阳师范学院生物与食品工程学院,安徽阜阳236041 [2]五邑大学信息工程学院,广东天门529020 [3]河南农业职业学院现代农业工程系,河南中牟451460
出 处:《安徽农业科学》2015年第5期27-28,34,共3页Journal of Anhui Agricultural Sciences
基 金:安徽省高校省级自然科学基金重点项目(KJ2014A194)
摘 要:[目的]研究恢复系SSSL W23-07-06-01-09中的恢复基因Rf3和Rf4对于野败型不育细胞质的遗传模式。[方法]以野败型不育系博白A为母本,恢复系SSSLW23-07-06-01-09(Rf3Rf3/Rf4Rf4)为父本杂交,采用分子标记辅助选择和连续回交的方法构建BC3F2群体,从中选择携带基因型Rf3Rf3/Rf4Rf4、rf3rf3/Rf4Rf4的单株,考察其花粉和小穗育性。[结果]恢复系SSSLW23-07-06-01-09中的恢复基因对于WA型不育系博白A表现出质量-数量性状的遗传。在恢复系SSSLW23-07-06-01-09中,除主效恢复基因Rf3和Rf4外,微效基因或者修饰基因也表现出对于博白A的恢复性作用,且效应较大。[结论]为水稻杂种优势研究和利用提供理论依据。[ Objective ] To analyze the genetic mode of restorer genes Rf3 and Rf4 for the WA-CMS system. [ Method ] The BC3F2 population, possessing the genetic background of the SSSLW23-07-06-01-09, was generated from the crosses between the SSSLW23-07-06-01-09 (recurent parent) and the WACMS line of BobaiA by backerossing and marker-assisted selection. In the BC3F2 population, the plants carrying the Rf3Rf3/Rf4Rf4 ,rf3ri3/Rf4Rf genotype were selected, their phenotyping for fertility in pollen and spikelet were evaluated. [ Result] The results showed that two pairs of dominant genes governed restoration of pollen fertility restoration, and some modifying or minor genes were in- volved in the inheritance of restorer ability besides Rf3 or Rf4 in SSSLW23-07-06-01-09, indicating that the genetic mode of Rf genes showed a qua]itatlve-quantltatlve characteristic for WA and DA-CMS system. [ Conclusion ] The study provided a theoretical basis for the study and use of heterosis in rice.
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