Pneumocandin B_0高产菌株选育  

Screening for high pneumocandin B_0 producing strain

作　　者：关亚鹏[1] 张莉[1] 刘靓[1] 赵静[1] 娄忻[1] 

机构地区：[1]华北制药集团新药研究开发有限责任公司微生物药物国家工程研究中心河北省工业微生物代谢工程技术研究中心,石家庄050015

出　　处：《中国抗生素杂志》2015年第2期88-90,95,共4页Chinese Journal of Antibiotics

摘　　要：目的筛选获得pneumocandin B0高产菌株。方法以pneumocandin B0产生菌PB22-79作为出发菌株,采用微波诱变方法处理,然后利用He-Ne激光复合制霉菌素抗性筛选菌种,以菌种发酵液效价作为筛选指标。结果获得一株高产突变株FH-24-135,其摇瓶生产能力比出发菌株提高了56.3%。且遗传特性稳定。研究确定了微波辐射的最佳时间为80s,而He-Ne激光复合制霉菌素抗性筛选的最佳剂量为90min和25μg/m L(MIC)。结论 微波诱变结合He-Ne激光复合制霉菌素抗性筛选方法是筛选pneumocandin B0产生菌的有效方法 ,有利于提高菌株的发酵水平,为工业化生产奠定基础。Objective Screening for a high pneumocandin B0 producing strain. Methods The primitive strain PB22-79 was treated with microwave mutation, and then followed by with He-Ne laser and nystatin-resistance. The titer of fermentation was choosed as screening index. Results A pneumocandin B0 high yield strain FH-24-135 was obtained and its flsak fermentation productivity was 56.3% higher than the primitive strain. The research indicated that the best microwave mutation time was 80s, the best He-Ne irradiation time was 90min, and the MIC of nystatin was 25μg/mL. Conclusion Microwave mutation followed with He-Ne laser compound nystatin-resistance is effective for screening high-yeilding mutants. The productivity increase of mutation strain established foundation ofpneumocandin B, on industrial scale.

关 键 词：Pneumocandin B0 复合诱变 HE-NE激光 抗性筛选 

分 类 号：R696[医药卫生—泌尿科学]