黔产粗毛淫羊藿与黔岭淫羊藿总黄酮对2型糖尿病小鼠IR相关因子的影响  

Effects of Total Flavonoe of Epimedium of Two Species in Guizhou on IR-related Factors in Type 2 Diabetes

作　　者：卢启振 张静 王悦云[1] 黄聪[1] 邓炜[1] 

机构地区：[1]贵阳中医学院,贵阳550002 [2]徐州市钟吾卫校药理教研室,江苏徐州221400

出　　处：《中国实验方剂学杂志》2015年第3期96-99,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81360624);贵州省中药现代化项目(黔科合ZY字[2013]3007号);贵州省科技厅和贵阳中医学院联合资金项目(黔科合中药字(2010)LKZ7026号);贵州省中医药管理局中药民族药科研项目(QZYY2010-004)

摘　　要：目的:研究黔产粗毛淫羊藿与黔岭淫羊藿中总黄酮(TFE)对四氧嘧啶(ALX)诱发2型糖尿病模型小鼠胰岛素抵抗(IR)相关因子的影响。方法:雄性小鼠100只,随机取10只为正常组,喂养基础饲料;其余的采用高脂饲料喂养小鼠6周,单次ip 2%ALX(200 mg·kg-1),建立2型糖尿病小鼠模型。将糖尿病模型小鼠随机分为6组,每组10只,分别为模型组,二甲双胍(150 mg·kg-1)组,两种TFE分别按剂量100,50 mg·kg-1分组,ig给药14 d。于末次给药后取血,检测小鼠血清胰岛素(FINS)、C肽水平;检测小鼠空腹血糖(FBG),注射胰岛素(Ins)40 min后血糖(BG);计算胰岛素耐量(ITT),胰岛素抵抗指数(HOMA-IR)和胰腺指数;观察小鼠胰腺形态学改变。结果:与正常组比较,模型组小鼠血糖升高非常显著(P<0.01),空腹血清中C肽,FINS有明显增加(P<0.05),胰腺组织损伤极为严重(P<0.01),胰腺指数降低非常显著(P<0.01)。与模型组相比,两种淫羊藿TFE均能够较明显地改善模型小鼠ITT(P<0.05),降低模型小鼠血清C肽、FINS含量及HOMA-IR(P<0.05),提高模型小鼠的胰腺指数(P<0.05),明显地修复模型小鼠的胰腺组织损伤(P<0.05)。两种TFE同剂量组相比,以粗毛淫羊藿TFE作用较为显著(P<0.05,P<0.01)。结论:两种黔产淫羊藿TFE对ALX诱生的2型糖尿病模型小鼠能增加胰岛素敏感性,较明显地改善模型小鼠胰岛素抵抗相关因子,保护受损的胰腺组织。以粗毛淫羊藿TFE作用较为明显,这可能与粗毛淫羊藿TFE中淫羊藿苷(ICA)含量高有较大关系。Objective： To study the effects of total flavonoe of Epimedium （TFE） from Epimedium acuminatum and E. Leptorrhizum grown in Guizhou on insulin resistance （IR） -related factors in mice induced by alloxan （ALX）. Method： Hundred male mice were divided into normal group and model group. The mice in model group were fed with high-fat diet for 6 weeks, then single intraperitoneal injection of 2% ALX solution （200 mg ·kg^-1 ） was made to establish type 2 diabetes models. The modeling mice were randomly divided into 6 groups with 10 in each group, including metformin group （150 mg ·kg^-1） , E. acuminatum TFE group （100, 50 mg·kg^-1） , E. leptorrhizum TFE group （100, 50 mg ·kg^-1） , respectively, once a day for 14 days. After 14 days, the animals were fasted for 12 hours, and killed. Then the level of serum insulin （FINS） and C peptide was determined. The blood sugar （FBG） and BG after injection of ins 40 min were detected. Insulin tolerance （ITT）, insulin resistance index （HOMA-1R） and pancreatic index were calculated. HE staining was used to observe the morphologic changes of pancreas in mice. Result： Compared with the normal group, blood sugar of the model group increased significantly （P 〈 0.01 ） , C-peptide and FINS significantly increased （ P 〈 0.05 ） , pancreatic tissue injury was serious （ P 〈 0.01 ） , the pancreas index decreased significantly （P 〈 0.01 ）. Compared with model group, two TFE from E. acuminatum all obviously improved ITT （P 〈 0.05）, pancreatic index （P 〈 0.05） , reduced C-peptide, FINS and HOMA-IR （P 〈 0.05） and obviously alleviated pancreatic tissue injury （P 〈 0.05） in mice induced by ALX. TFE from E. acuminatum had more obvious effect （P 〈 0.05, P 〈 0.01 ）. Conclusion： Two kinds of TFE from E. acuminatum have certain effect on the type 2 diabetes mice induced by ALX, and can increase insulin sensitivity, improve IR-related factors on the type 2 diabetes mice, and have a good protective

关 键 词：粗毛淫羊藿 黔岭淫羊藿 淫羊藿总黄酮 四氧嘧啶 2型糖尿病 胰岛素抵抗 

分 类 号：R285.5[医药卫生—中药学]