健脾养正消癥方对裸鼠胃癌移植瘤及肝转移灶的生长转移及相关基因的影响  

Effects on Growth and Related Genes of Jianpi Yangzheng Xiaozheng Decoction in Gastric Cancer Xenografts and Liver Metastases Mice

作　　者：王瑞平[1] 朱超林[1] 刘包欣子[1] 朱云涛[2] 唐晓龙[2] 邹玺[1] 

机构地区：[1]南京中医药大学附属医院,南京210029 [2]南京中医药大学,南京210046

出　　处：《中国实验方剂学杂志》2015年第3期164-168,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家中医临床研究基地业务建设科研专项(JDZX2012087;JDZX2012086)

摘　　要：目的:观察健脾养正消癥方对裸鼠胃癌移植瘤及转移灶的生长转移及相关基因的影响。方法:建立人胃癌裸鼠移植瘤模型,并随机分为模型组,阳性组(5-氟尿嘧啶,5-Fu组),健脾养正消癥方低、高剂量组。观察健脾养正消癥方对裸鼠胃癌移植瘤的抑瘤率及肝转移灶的情况。反转录酶-聚合酶链锁反应(RT-PCR)法检测健脾养正消癥方对胃癌原位移植瘤及肝转移灶中基质金属蛋白酶2(MMP-2),MMP-9,MMP-14,血管内皮生长因子(VEGF),RECK基因表达的影响。Western blot法检测健脾养正消癥方对胃癌原位移植瘤及肝转移灶中P-STAT3,MMP-2,MMP-9,MMP-14,VEGF,RECK基因表达的影响。结果:健脾养正消癥方高、低剂量组小鼠瘤体平均瘤重明显小于模型组(P<0.01)。健脾养正消癥方作用于裸鼠胃癌移植瘤及其肝转移灶后,侵袭转移相关基因中MMP-2,MMP-9,MMP-14,VEGF表达下降,RECK基因表达上升;健脾养正消癥方作用于裸鼠胃癌移植瘤及其肝转移灶后,其相关蛋白P-STAT3,MMP-2,MMP-9,MMP-14,VEGF表达下降,RECK蛋白表达上升。结论:健脾养正消癥方对裸鼠胃癌原位移植瘤及肝转移灶生长具有抑制作用;健脾养正消癥方能够下调P-STAT3,MMP-2,MMP-9,MMP-14,VEGF等相关基因或蛋白的表达同时上调RECK基因或蛋白的表达。Objective： To investigate the effects of Jianpi Yangzheng Xiaozheng decoction （JYXD） on gastric cancer growth, liver metastasis situation and metastasis-related genes. Method： The human gastric cancer model in nude mice was established and the mice were randomly divided into control group, 5-Fu group, high-and low-dose JYXD groups. The inhibition rate of gastric cancer xenografts and liver metastases situation in nude mice were observed. The gene expressions of matrix metalloproteinases 2 （MMP-2）, MMP-9, MMP-14, vascular endothelial growth factor （VEGF） and reversion inducing cysteine-rich protein with Kazal motifs （RECK） were detected by transcription-polymerase chain reaction （RT-PCR） assay. The protein expressions of signal transducer and activator of transcription3 （STAT3）, MMP-2, MMP-9, MMP-14, VEGF and RECK were detected by Western Blot assay. Result： The average tumor weight was lower, the expressions of invasion and metastasis-related genes of MMP-2, MMP-9, MMP-14, VEGF decreased, the gene expression of RECK rised, the expression of invasion and metastasis-related proteins P-STAT3, MMP-2, MMP-9, MMP-14, VEGF decreased, and the protein expression of RECK rised in all dose JYXD groups as compard with the control group （P 〈 0. 01 ）. Conclusion： JYXD could inhibite the growth of gastric cancer xenografts liver metastase and down-regulate the expressions of P-STAT3, MMP-2, MMP-9, MMP-14, VEGF genes or proteins and upregulate the expressions of RECK gene or protein.

关 键 词：健脾养正消癥方 裸鼠胃癌移植瘤 肝转移灶 侵袭转移 

分 类 号：R285.5[医药卫生—中药学]