麦粒灸对双转基因阿尔茨海默病小鼠额叶皮层及海马区Aβ_(1-42)表达的影响  

Effects of grain-sized moxibustion on expression of Aβ_(1-42) in prefrontal cortex and hippocampus in double-transgenic AD mice

作　　者：包烨华[1] 张永生[2] 楚佳梅[1] 曾友华[1] 李丽萍[1] 高灵爱[3] 余静[1] 

机构地区：[1]杭州市中医院针灸科,浙江杭州310007 [2]浙江中医药大学,浙江杭州310053 [3]杭州市余杭区第五人民医院

出　　处：《中国针灸》2015年第1期59-65,共7页Chinese Acupuncture & Moxibustion

基　　金：浙江省自然科学基金项目:LY13H270002;杭州市科技发展计划项目:2011KJJ16

摘　　要：目的:探讨在阿尔茨海默病(Alzheimer’s disease,AD)病理早期采用麦粒灸"心俞""肾俞"穴对转基因AD小鼠的治疗作用。方法:采用PCR法鉴定淀粉样前体蛋白/早老素1(APP/PS1)双转基因AD传代小鼠的基因表型,选取1.5月龄雌性转基因阳性Tg6799小鼠17只,随机分为模型组(9只)和治疗组(8只),同龄、同背景的C57BL/6J野生型雌性小鼠9只作为正常组。治疗组取双侧"心俞""肾俞"穴行麦粒灸治疗,每日1次,10次为一疗程,共治疗9个疗程。模型组、正常组均给予抓取、固定及放置未燃烧的艾炷等刺激。应用Morris水迷宫中逃避潜伏期、穿越平台的次数及目标象限停留时间百分比来评价小鼠的学习记忆能力,苏木精-伊红(HE)染色法观察小鼠脑组织形态学的改变,免疫组化法检测小鼠额叶皮层及海马区β-淀粉样蛋白1-42(β-amyloid protein,Aβ1-42)的表达水平。结果:经麦粒灸法治疗后,治疗组小鼠学习记忆能力提高,与模型组比较,逃避潜伏期缩短,穿越平台次数增多,目标象限停留时间延长(均P<0.05);治疗组穿越平台次数及目标象限停留时间与正常组比较差异无统计学意义(均P>0.05)。与正常组相比,模型组AD小鼠大脑额叶皮层、海马区Aβ1-42表达阳性面积和总光密度明显增高(均P<0.01);与模型组相比,治疗组大脑额叶皮层、海马区Aβ1-42表达阳性面积和总光密度明显减少(P<0.01,P<0.05)。结论:麦粒灸"心俞""肾俞"穴可显著改善APP/PS1转基因AD小鼠的学习记忆能力,抑制Aβ1-42的过度表达、聚集。Objective To explore the therapeutic effect of grain-sized moxibustion at ＂Xinshu＂ （BL 15） and ＂Shenshu＂ （BL 23） on early-stage Alzheimer＇s disease （AD） in transgenosis AD mice. Methods The genotyping of amyloid precursor protein/presenilin I（APP/PS1） doubletransgenic AD mice were detected by PCR method. Seventeen 1.5-month female transgenic （Tg 6799） mice were randomly divided into a model group （9 cases） and a treatment group （8 cases）. Nine female C57BL/6J wild-type mice with identical age and background were selected into a normal group. The treatment group was treated with grain-sized moxibustion at bilateral ＂Xinshu＂ （BL 15） and ＂Shenshu＂ （BL 23）, once a day, ten treatments were considered as one course, and total 9 courses were given. The model group and normal group were treated with stimulus such as grabbing, immobilization and non-ignited moxa cone. Morris water maze （escape latency, crossing times and dwell time in the target quadrant） was applied to evaluate the learning and memory ability. Hematoxylin-Eosin （HE） staining was used to observe morphology changes in the brains of AD mice. β-amyloid protein 1-42 （Aβ1-42） in the area of prefrontal cortex and hippocampus was detected by immunohistochemical method. Results After the treatmenl of grain-sized moxibustion, learning and memory ability in the treatment group was increased; compared with the model group, the escape latency was shorten, crossing times was increased, and dwell time in the target quadrant was prolonged （all P〈0. 05）. The crossing times and dwell time in the target quadrant in the treatment group were not significantly different fromthose in the normal group （both P〉0.05）. Compared with the normal group, the positive area and integral optical density of Aβ1-42 in prefrontal cortex and hippoeampus in the model group were increased （all P〈0.01）. Compared with the model group, the positive area and integral optical density of Aβ1-42 in prefrontal cort

关 键 词：阿尔茨海默病 APP/PS1转基因鼠 麦粒灸 穴 心俞 穴 肾俞 Aβ1-42 

分 类 号：R246.1[医药卫生—针灸推拿学]