一株H10N7亚型禽流感病毒的分离鉴定及生物学特性研究  

Biological characteristic of an H10N7 avian influenza virus isolated from Wood Sandpiper in Qinhuangdao in 2013

作　　者：王德丽[1] 慈彦鹏[1] 崔艳芳[1] 樊兆斌[1] 刘丽玲[1] 田国彬[1] 曾显营[1] 陈化兰[1] 李雁冰[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/农业部动物流感重点开放实验室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2015年第1期6-9,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：农业部公益性行业专项-边境地区动物疫病防控技术体系研究(201103008);农业部动物流感监测与防治专项

摘　　要：2013年,从秦皇岛野鸟林鹬体内分离到一株H10N7亚型禽流感病毒(AIV),命名为A/Wood Sandpiper/Qinhuangdao/660-662/2013(H10N7)[简称WSP/QHD/660-662/2013(H10N7)]。本研究对该分离株的全基因序列进行测定,并对其进行致病性研究。基因组序列分析表明:该病毒的HA蛋白裂解位点为334PELMQGRGL343,属于低致病性AIV的分子特征,其HA基因与A/Duck/Hunan/S11205/2012(H10N3)的相似性达到97.90%,NA基因与A/Domestic Duck/Republic of Georgia/1/2010(H10N7)的相似性达到97.46%,内部基因与H9N2等多亚型AIV的相应基因节段具有较高的相似性,推测该分离株可能为一株多亚型流感病毒的重组株。对动物致病性试验结果显示:该病毒可以感染哺乳动物模型BALB/c小鼠,并且仅能够在鼠的肺脏和鼻甲骨粘膜上皮细胞中复制,表明该病毒分离株对小鼠也呈现低致病性。An H10N7 subtype avian influenza virus （AIV） WSP/QHD/660-662/2013 （H10NT） was isolated from life Wood Sandpiper in Qinhuangdao, Hebei province in 2013, and the virus was subject to whole genomic sequencing and pathogenic test in mammalian model of BALB/c mice. The sequence analysis of HA showed that the virus possessed a motif of 33PELMQGRGL343 at the cleavage site of HA protein which categorized the isolate as a low pathogenic A1V. In addition, the HA gene of the isolate shared the highest homologous （97.90%） to that of the AIV A/Duck/HunardSll205/2012（H10N3）, but the identity of NA gene was the highest （97.46%） to the AIV A/Domestic Duck/Republic of Georgia/1/2010（H10N7）. While, the internal genes of the isolate were higher identity to H9N2 and the other subtype AIVs, respectively. The sequence alignment revealed that the isolate probably was a natural reassortant from different subtype AIVs. Pathogenic study displayed that the artificially infected mice did not show any clinical symptoms and weight gain loss. However, the virus could be only detected in lungs and turbinates of the infected mice. These data demonstrated that the virus was also low pathogenic to mice.

关 键 词：禽流感病毒 H10N7 进化分析 致病性 

分 类 号：S852.65[农业科学—基础兽医学]