京海黄鸡杂交配套系亲本两种球虫疫苗免疫效果的比较  

Comparison of immune effects of two coccidiosis vaccines in Jinghai Yellow chicken cross-breeding system parents

作　　者：林雨鑫[1,2] 孙明明[1,2] 戴国俊[1,2] 张菁菁[1,2] 张跟喜[1,2] 谢恺舟[1,2] 王金玉[1,2] 宋野[1] 王永娟[3] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]江苏省动物遗传繁育与分子设计重点实验室,江苏扬州225009 [3]江苏京海禽业集团有限公司,江苏海门226103

出　　处：《中国预防兽医学报》2015年第1期67-70,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家肉鸡产业技术体系项目(nyctx-42-G1-05);江苏省产学研创新资金前瞻性联合研究项目(BY2013063-06);江苏省高校自然科学研究重大项目(11KJA230001);江苏高校优势学科建设工程项目(PAPD);扬州大学新世纪人才工程项目资助

摘　　要：为评价两种球虫疫苗对京海黄鸡杂交配套系亲本良凤鸡(BB鸡)和岭南黄鸡(FF鸡)的免疫效果,本研究对9日龄实验鸡接种两种疫苗[500个孢子化卵囊(V1组)、正典球虫苗(V2组)],24 d后攻柔嫩艾美耳球虫(E.tenella)2.5万个/只,攻虫后8 d检测各项评价指标。结果显示:攻虫后临床表现、盲肠病变、相对增重率及抗球虫指数(ACI),BB鸡优于FF鸡,V1组优于V2组;两鸡群血浆中过氧化氢酶(CAT)、超氧化物歧化酶(SOD)的含量V1组均显著高于V2组(p<0.05);BB鸡γ干扰素(IFN-γ)含量V1组显著高于V2组(p<0.05);FF鸡血浆中β胡萝卜素(β-C)含量V1组显著高于V2组(p<0.05)。综合分析表明,BB鸡E.tenella抗性优于FF鸡,500个E.tenella活卵囊免疫效果优于正典球虫疫苗。In this study, Jinghai Yellow chicken cross-breeding system parental BB and FF were vaccinated two vaccines of 500 live Eimen＇a tenella sporulated oocysts （V1） and standard coccidiosis vaccine （V2） at 9-day-old, and challenged with 2.5×10^4 sporulated E.tenella oocysts at 24 days post vaccination （PV）. Then, all evaluation parameters were examined at 8 days post challenge （PC） for evaluating the immune effects of two coccidiosis vaccines in BB and FF. The results showed that BB chickens were not serious to FF chickens in clinical signs, lesions in the cecum, relative growth rate, and ACI values post PC. And also the vaccine V1 was more efficient against coccidiosis than that of vaccine V2. The plasma parameters of CAT, SOD in V1 group were significantly higher than that of V2 group （p〈0.05）. For BB, the IFN-γ levels in group V1 were significantly higher than that in group V2 （p〈0.05）. As for FF chickens, the β-C levels in group V1 were significantly higher than that in group V2 （p〈0.05）. Comprehensive analysis shows that the E.tenella resistance of BB chickens was superior to FF chickens, the immtme effect of group V1 was superior to group V2.

关 键 词：BB鸡 FF鸡 柔嫩艾美耳球虫 疫苗 

分 类 号：S852.7[农业科学—基础兽医学]