青蒿琥酯对高糖诱导的肾小管上皮细胞凋亡及TNF-α、IL-8表达的影响  

Effects of artesunate on high glucose-induced cell apoptosis, TNF-α and IL-8 expression in renal tubular epithelial cells

作　　者：蒋姗姗[1] 龙艳[1] 苏珂[1] 聂寒 黄漓莉[1] 杨帆[1] 李争明[1] 荀靖琼[1] 

机构地区：[1]桂林医学院附属医院内分泌科,541004

出　　处：《天津医药》2015年第1期20-24,共5页Tianjin Medical Journal

基　　金：广西医疗卫生适宜技术研究与开发项目(S201316-07);广西自然科学基金项目(2012GXNSFAA053085);广西高等学校计划科研项目(201204LX246)

摘　　要：目的探讨青蒿琥酯(Art)对高糖诱导的大鼠肾小管上皮细胞(NRK-52E)的凋亡及肿瘤坏死因子-α(TNF-α)、白细胞介素-8(IL-8)表达的影响。方法传代培养大鼠肾小管上皮细胞,分为正常对照组、高糖组、高糖+不同浓度青蒿琥酯组(10、20、30 mg/L)、高糖+依那普利对照组(5 mg/L)。四甲基偶氮唑盐微量比色(MTT)法观察细胞增殖能力的改变;流式细胞仪Annexin V-FITC/PI双染法检测细胞凋亡指数;酶联免疫吸附(ELISA)法检测细胞培养上清液中TNF-α和IL-8蛋白含量。结果 (1)经高糖处理48 h后,NRK-52E细胞增殖抑制,细胞凋亡率增高,细胞上清液中TNF-α、IL-8蛋白浓度升高。(2)经青蒿琥酯干预后,与高糖组比较,NRK-52细胞增殖明显,细胞凋亡率减低,细胞上清液中TNF-α、IL-8蛋白浓度降低,其干预作用呈现剂量依赖性。结论青蒿琥酯可抑制高糖诱导的NRK-52E细胞凋亡及细胞上清液中炎症因子TNF-α、IL-8的表达。青蒿琥酯的抗炎和免疫调节作用可能有助于糖尿病肾病的治疗。Objective To investigate the effects of artesunate （Art） on cell apoptosis, tumor necrosis factor-alpha （TNF-α） and interleukin-8 （IL-8） expression induced by high glucose in rat renal tubular epithelial cells （NRK-52E）. Methods NRK-52E cells were cultured and divided into normal control group, high glucose group, high glucose with different concen？trations of Art （10 mg/L, 20 mg/L and 30 mg/L） groups, and high glucose with Ena （5 mg/L） group. MTT assay was used to de？tect the cell proliferation. The apoptotic rate was evaluated by flow cytometry with AnnexinV-FITC/PI double stains. The pro？tein levels of TNF-αand IL-8 in the cell culture supernatant were determined using ELISA. Results High glucose inhibit？ed NRK-52E proliferation, induced its apoptosis, and the expressions of TNF-αand IL-8 in the supernatant. Application of Art obviously abolished the effects of high glucose, and the effects of Art were showed in dose-dependent manner. Conclu？sion Art can suppress high glucose-stimulated cell apoptosis, enhance TNF-αand IL-8 expression in NRK-52E cells. The anti-inflammatory action and immune regulation of Art could be a novel approach of treating diabetic nephropathy.

关 键 词：糖尿病肾病 细胞凋亡 肿瘤坏死因子α 白细胞介素8 青蒿琥酯 肾小管上皮细胞 

分 类 号：R587.24[医药卫生—内分泌]