17β-雌二醇对SiO_2刺激的小鼠肺成纤维细胞表达Caveolin-1和Ⅲ型胶原的影响  

Effects of 17β-estradiol on the Expression Caveolin-1 and Type Ⅲ Collagen in the Mouse Lung Fibroblast Stimulated by SiO_2

作　　者：肖永红[1] 梁晓飞[1] 王悦[1] 肖倩[1] 

机构地区：[1]河北联合大学公共卫生学院,唐山063000

出　　处：《四川大学学报（医学版）》2015年第1期62-64,共3页Journal of Sichuan University(Medical Sciences)

基　　金：河北省科学技术研究与发展计划(No.10276113D);河北省教育厅课题(No.2006139);唐山市科技计划项目(No.13130269z)资助

摘　　要：目的探讨不同浓度17β-雌二醇(17β-E2)对二氧化硅(SiO2)刺激的小鼠肺成纤维细胞表达Caveolin-1和Ⅲ型胶原的影响。方法将体外培养的小鼠肺成纤维细胞分5组:空白对照组,SiO2(100mg/L)组,SiO2(100mg/L)+不同剂量17β-E2组(10-8、10-7、10-6 mol/L),17β-E2作用48h后收集各组细胞,应用免疫组化法检测Caveolin-1和Ⅲ型胶原表达。结果 SiO2处理组小鼠肺成纤维细胞与空白对照组比较Caveolin-1表达降低(P<0.05),而SiO2+17β-E2处理组随着17β-E2剂量增大Caveolin-1表达明显升高(P<0.05);各处理组Ⅲ型胶原表达差异有统计学意义(P<0.05),SiO2组与空白对照组比较增高(P<0.05),而17β-E2处理组均低于SiO2组(P<0.05);相关分析显示,17β-E2与Caveolin-1的表达呈正相关(r=0.926,P<0.05),与Ⅲ型胶原表达呈负相关(r=-0.914,P<0.05),Caveolin-1与Ⅲ型胶原表达亦呈负相关(r=-0.887,P<0.05)。结论 17β-E2可能通过上调肺成纤维细胞Caveolin-1的表达,抑制Ⅲ型胶原表达而发挥抗纤维作用。Objective To observe the effects of different concentration of 17β-estradiol （17β-E2） on the expression of Caveolin-1 and type Ⅲ collagen in the mouse lung fibroblast stimulated by SiO2. Methods Fibroblast cells were devided into five groups： blank control group, SiO2 （100 ng/L） group and SiO2 （100 mg/L）＋ different concentration of 17β-E2 （10^-8 ,10^-7,10^-6 mol/L） groups. After treated with different concentration of 17βEz, for 48 h, the cells were collected, then the expression of Caveolin-1 and of type Ⅲ collagen were examined with immunohistochemistry method. Results Compared with the blank control group, the expression of Caveolin-1 in mouse lung fibroblasts treated by SiO2 significantly decreased （P〈0.05）, While which in the SiO2 q＋17β-E2 group significantly increased as 17β -E2 dose increased （P〈 0.05） ; There were significantly different in the expression of typeⅢl collagen among different groups （P〈0.05）, which in SiO2 group were evidently higher than that in the blank control group, while which in 17β-E2 groups decreased significantly, when compared with SiO2 group （P〈 0.05） ; 17β-E2 increased the expression of Caveoliml and decreased the expression of typeⅢ collagen in the dose dependent manner. Correlation analysis showed that 17β-E2 was positive correlated with the expression of Caveolin- 1 （r=0. 926, P〈0.05）, and negative correlated with the express of type Ⅲ collagen （r= -0. 914, P〈0.05）, and the expression of Caveolin-1 and the expression of type Ⅲcollagen was negatively correlation （r= 0. 887, P〈 0.05）. Conclusion 17β-E2 may inhibit the expression of type Ⅲ collagen by upregulating the expression of Caveolin-1 in mouse lung fibroblast cell to play a role in the resistance of the lung fibrosis.

关 键 词：17Β-雌二醇 肺成纤维细胞 CAVEOLIN-1 Ⅲ型胶原 

分 类 号：R563.9[医药卫生—呼吸系统]