GNB3 C825T和ACE I/D多态性相互作用与子痫前期关系的研究  

Interaction Between GNB3 C825T and ACE I/D Polymorphisms in Pre-eclampsia

作　　者：马蕾[1] 范平[1] 刘兴会[2] 何国琳[2] 刘瑞[3] 任荣梅[1] 陈一虹[1] 刘宇[4] 白怀[1] 

机构地区：[1]四川大学华西第二医院遗传代谢性疾病及围生医学实验室,成都610041 [2]四川大学华西第二医院妇产科,成都610041 [3]四川大学华西医院人类疾病相关多肽研究室,成都610041 [4]四川大学华西基础医学与法医学院生物化学与分子生物学教研室,成都610041

出　　处：《四川大学学报（医学版）》2015年第1期118-122,2,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(No.39870749)资助

摘　　要：目的探讨G蛋白β3亚单位（GNB3）和血管紧张素Ⅰ转换酶（ACE）基因相互作用是否与子痫前期的发生有关联。方法采用ACE基因PCR扩增片段长度多态性及GNB3基因限制性片段长度多态性方法,对成都地区汉族188例子痫前期及273例正常孕妇进行对照检测。结果子痫前期及正常孕妇GNB3C825T和ACE I/D位点基因型和等位基因频率在两组之间差异无统计学意义（P〉0.05）,未见GNB3C825T等位基因及ACEI/D相互作用与子痫前期发生的危险增加有关（OR0.439~1.203,P均〉0.05）。对联合基因型与血压水平关系的分析发现,正常对照组TT/II基因型携带者其舒张压水平增高〔（77.61±1.26）mmHg（1mmHg=0.133kPa）〕,且高于TT/ID〔（70.94±1.64）mmHg〕、CT/ID〔（73.15±0.89）mmHg〕和CT/DD〔（72.57±2.14）mmHg〕基因型携带者（P均〈0.05）;未见患者组联合基因型与收缩压和舒张压水平有关（P〉0.05）。结论本研究未发现GNB3基因C825T及ACE基因I/D位点的相互作用与中国人（成都地区）子痫前期发病有关联,但发现两基因的相互作用与正常孕妇舒张压水平有关。Objective To verify the hypothesis if interaction between the G protein 133 subunit （GNB3） C825T polymorphism and angiotensin-I converting enzyme （ACE） insertion/deletion （I/D） could lead to the increased risk of pre-eclampsia. Methods Analyses of ACE and GNB3 genotypes were performed in 188 pre- eclamptic patients and 273 normal pregnant controls by polymerase chain reaction （PCR） and PCR-restriction fragment length polymorphism in Chinese population, respectively. Results The distributions of alleles and genotypes for the GNB3 C825T and ACE I/D polymorphisms were not found to be significantly associathcd with pre-eclamptic status. No significant interaction of the influence of GNB3 T allele and ACE genotypes on the risk of pre-eelampsia was observed （OR 0. 439-1. 203, all P 〉 0. 05）. However, we found that in homozygous 825T genotype carriers with the ACE Ⅱ genotype in controls diastolic blood pressure （DBP） levels showed highest [（77.61±1.26） mmHg （1 mmHg= 0. 133 kPa）] among other three genotype combinations [TT/ID, （70.94 ±1.64） mmHg; CT/ID, （73.15±0.89） mmHg; CT/DD, （72.57±2. 14） mmHg] （all P〈0.05）. No significant effect on systolic blood pressure （SBP） or DBP levels in the patients were observed. Conclusion Our data suggest no significant interaction of the GNB3 825T allele carriers with the ACE I/D polymorphism in pre-eclampsia in Chinese population in Chengdu area. However there is the interaction of the two genes on DBP levels in pregnancy women without pre-eclampsia in the population.

关 键 词：子痫前期 基因多态性 GNB3 ACE 

分 类 号：R714.244[医药卫生—妇产科学]