半滑舌鳎性别决定的QTL互作研究  

QTL Interaction of Sex Determination in Half-smooth Tongue-sole(Cynoglossus semilaevis)

作　　者：王景伟[1,2] 宋文涛[3] 蒋丽[2] 陈松林[3] 李恒德[2] 

机构地区：[1]福建农林大学动物科学学院,福州350002 [2]中国水产科学研究院生物技术研究中心,北京100141 [3]中国水产科学研究院黄海水产研究所,青岛266071

出　　处：《农业生物技术学报》2015年第1期89-95,共7页Journal of Agricultural Biotechnology

基　　金：国家高技术研究发展计划(863计划)(No.2012AA10A402);中央级公益性科研院所基本科研业务费专项资金(No.2013A0401;No.2013A0506)

摘　　要：半滑舌鳎(Cynoglossus semilaevis)成体雌雄差异较大,性别控制是提高半滑舌鳎养殖效益的有效手段,而实现性别控制的关键在于揭示半滑舌鳎的性别决定的遗传机制。半滑舌鳎的遗传性别决定方式为ZW型,这种性别决定方式可能存在多因子剂量效应,其性别决定不仅与性染色体有关,常染色体也会参与性别调控,遗传性别是性染色体和常染色体的协同作用的结果。本研究从数量性状基因座(quantitative trait loci,QTL)互作的角度来探讨半滑舌鳎遗传性别决定机制,将性别作为二项性状,利用半滑舌鳎高密度微卫星遗传连锁图谱,采用logistic回归模型扫描所有标记间的二阶组合对性别是否存在影响。研究结果发现,共有5个标记组合在P<0.05显著水平上被检测出来,其中组合SCAFFOLD149_7459和SCAFFOLD7854_71905在P<0.01水平上显著;5个交互组合位于7个常染色体的9个标记当中,这些标记主要集中在连锁群2、10和11,其中连锁群10-11发现了两个差异显著的交互对,且两个交互对均有SCAFFOLD149_7459,说明SCAFFOLD149_7459可能在互作过程中具有较大的影响。研究表明这些标记附近的基因参与了半滑舌鳎的性别决定,也即常染色体可能参与其性别决定的过程,这对进一步研究半滑舌鳎的性别决定和性别控制具有重要意义。Adult half-smooth tongue-sole （Cynoglossus semilaevis） differs widely in size between male and female, and sex control is an effective measure to improve economic profit for half-smooth tongue-sole aquaculture and the key to achieving sex control is to reveal the genetic mechanism of sex determination. The pattern of half-smooth tongue-sole＇s genetic sex determination is ZW type. This kind of type may exist multifactor dose effect, that is, sex determination is not only related with sex chromosomes, but also autosomes may involve in the regulation of sex. Therefore, genetic sex is the result of sex chromosomes and autosomes＇ synergistic effect. In this study, we tried to investigate the mechanism of half-smooth tongue-sole＇ s sex determination from the perspective of quantitative trait loci （QTL） interaction. Sex was taken as a binary trait,all bi-marker interactions were scanned with logistic regression model by using of high density microsatellite genetic linkage map. Results showed that 5 bi-marker combinations were detected at significance level of P〈 0.05, among them, SCAFFOLD149_7459 and SCAFFOLD785471905 was significantly at P〈0.01 level, 5 bi-marker combinations came from 9 markers among 7 autosomes. These detected markers were mainly located on chromosome 2, 10 and 11, especially 2 bi-marker combinations located on linkage groups 10-11 and both possessed of SCAFFOLD149_7459, which indicating that this marker might have a greater impact on the process of interaction. This study showed that genes close to these markers were involved into sex determination of half-smooth tongue-sole, which means that autosomes may participate in the process of sex determination. The results have important meaning for further study sex determination and sex control of half- smooth tongue-sole.

关 键 词：半滑舌鳎 性别决定 数量性状基因座(QTL)互作 

分 类 号：S512.1[农业科学—作物学]