抗炎因子TSG-6抑制兔耳瘢痕增生的实验研究  

The experimental study of anti-inflammatory cytokine TSG-6 inhibits hypertrophic scar formation in rabbit ears model

作　　者：王晖[1] 李小静[1] 陈钊[1] 

机构地区：[1]安徽医科大学第一附属医院整形外科,合肥230022

出　　处：《安徽医科大学学报》2015年第1期45-49,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81272107)

摘　　要：目的通过建立兔耳增生性瘢痕模型,研究肿瘤坏死因子α刺激基因-6(TSG-6)在增生性瘢痕形成过程中的作用及机制。方法建立兔耳增生性瘢痕模型,右侧耳创面为实验组,注射TSG-6,左侧均注射等量PBS作为对照组,通过比较各组瘢痕指数(SEI)及Ⅰ、Ⅲ型胶原表达的不同来评价瘢痕增生程度的差异。采用免疫组化法及逆转录聚合酶链反应(RT-PCR)法检测炎症因子白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)及肿瘤坏死因子-α(TNF-α)在各组中的表达;以透射电镜观察及TUNEL法检测瘢痕组织成纤维细胞凋亡的变化。结果与PBS对照组比较,TSG-6治疗组炎症因子IL-1β、IL-6及TNF-α表达减少炎症反应明显减轻,瘢痕组织成纤维细胞凋亡明显增多,且SEI及Ⅰ、Ⅲ型胶原含量均低于对照组,差异有统计学意义(P<0.05)。结论抗炎因子TSG-6能明显减弱增生性瘢痕的形成,其对瘢痕形成的抑制作用可能与抑制炎症反应及促进成纤维细胞凋亡相关。Objective To observe the effect of tumor necrosis factorαstimulated gene-6 （ TSG-6 ） on hypertrophic scarring by using a rabbit ear model. Methods TSG-6 and PBS were injected intradermally in the right and left ear wounds, respectively. Collagen I and III expression detected by immunohistochemistry and scar elevation index （ SEI） was used to evaluate the extent of scarring. The expression of inflammatory factors interleukin-1β（ IL-1β） , interleukin-6 （ IL-6 ） and tumor necrosis factor-α（ TNF-α） was detected by immunohistochemistry and reverse tran-scription polymerase chain reaction. Transmission electron microscope （ TEM） and TUNEL analyses were used to detect fibroblast apoptosis. Results Compared with control scars, TSG-6-treated wounds exhibited decreased in-flammation significantly as evidenced by the lower levels of IL-1β, IL-6 , TNF-α. The apoptosis rate was higher and the SEI and the synthesis of collagens I and III were significantly decreased in the TSG-6-treated scars （ P〈0. 05 ） . Conclusion Immediate topical injection of TSG-6 during the wound healing process can reduce the severity of hy-pertrophic scarring in a rabbit model. The anti-cicatrix effect of TSG-6 may result from controlling inflammation, in-ducing fibroblast apoptosis and promoting collagen degradation.

关 键 词：动物模型 增生性瘢痕 兔 炎症 TSG-6 

分 类 号：R619.6[医药卫生—外科学]