流式细胞技术检测儿童哮喘患者抗FcepsilonRI自身抗体的研究  

Study on the detection of children with asthma refer to flow cytometry anti Fcepsilon RI autoantibody

作　　者：饶冬东[1] 张福辉 赵静[1] 毛剑芳[1] 

机构地区：[1]福建省妇幼保健院检验科,福建福州350001 [2]厦门市妇幼保健院检验科,福建厦门361000

出　　处：《临床医药文献电子杂志》2015年第2期234-235,共2页Electronic Journal of Clinical Medical Literature

摘　　要：目的近来研究发现过敏和自身免疫性病变之间或许存在联系,嗜碱性粒细胞和组织肥大细胞在哮喘的病理过程中发挥作用。本研究的目的是在儿童哮喘患者中检测FcεRI功能性自身抗体的对嗜碱性粒细胞的反应性,通过运用流式细胞嗜碱性粒细胞活化试验测定CD63的表达。方法从2012年1月-2014年6月,随机纳入于厦门市妇幼保健院儿科病房就诊,根据美国胸科协会制定的诊断指南诊断为哮喘的儿童,非过敏的健康志愿者的血清刺激患者组以及对照组以后,采用基于流式细胞仪的嗜碱性粒细胞活化试验检测方法,测定CD63的表达,因CD63的表达可用于识别嗜碱性粒细胞是否被患者或者健康对照组的血清激活。结果共纳入研究的哮喘儿童有72名,健康对照组32例。29/78（37.2%）的哮喘患者血清表达CD63＋的嗜碱性粒细胞,而在健康受试者的血清中只有4/32（12.5%）表达CD63＋嗜碱性粒细胞,在哮喘患者中CD63＋嗜碱性粒细胞的比例较健康受试者相比差异具有统计学意义（P〈0.05）。结论 FcεRI自身抗体可能与儿童哮喘的疾病相关。Objective Recent studies have found an association between autoimmune diseases and allergies may, basophils and tissue mast cells play a role in the pathogenesis of asthma. The purpose of this study is to test the functionality FcεRI autoantibodies in children with asthma against basophil reactivity, using the expression of CD63 was determined by flow cytometry basophil activation assay. Methods From January 2012 to June 2014,in the pediatric ward randomly into treatment of maternal and Child Health Hospital of Xiamen City, the asthma diagnosis was made according to American Thoracic Society diagnostic guidelines, after the stimulation of serum from nonallergic donors to patients and controlling groups, using flow cytometry based basophil activation assay method to detection expression of CD63, expression of CD63 can be help to make sure whether the basophils cells are activated in patient, or healthy controls. Results The study included a total of 72 children with asthma, healthy control group of 32 patients. 29/78（37.2%） in the serum of patients with asthma expression of CD63＋ basophils, and in the serum of healthy subjects, only 4/32（12.5%） the expression of CD63＋ basophils in patients with asthma CD63＋ percentage of basophils compared statistically significant（P〈0.05） compared with healthy subjects. Conclusion FcεRI autoantibodies may be disease-related asthma in children.

关 键 词：流式细胞技术 儿童哮喘 Fcepsilon RI自身抗体 

分 类 号：R556[医药卫生—血液循环系统疾病]