H7N9流感病毒HA、NA蛋白的抗原表位预测及其与HLA-Ⅱ类等位基因的相关性分析  

Prediction of antigenic epitopes on HA,NA amino acid sequences of novel influenza A( H7N9) virus and analysis association between susceptibility and HLA-Ⅱalleles

作　　者：刘雪婷[1] 王珊[1] 张俊艳[1] 刘兆宇[1] 陈惠芳[1] 邹泽红[1] 肖兰艳 及志恒 何颖[1] 

机构地区：[1]广州医科大学附属第二医院广东省过敏反应与免疫重点实验室/呼吸疾病国家重点实验室变态反应研究室,广州510260

出　　处：《中国免疫学杂志》2015年第1期16-21,共6页Chinese Journal of Immunology

基　　金：国家自然基金青年项目(81301948);广东高校优秀青年创新人才培养计划项目(20131LYM_0070);广州医科大学博士启动项目(2012C40)的资助

摘　　要：目的:分析H7N9流感病毒的HA、NA蛋白的抗原表位;预测H7N9流感病毒相关的HLA-Ⅱ类等位基因及易感人群。方法:软件分析HA、NA的同源性、B细胞表位、T细胞表位以及与HA、NA结合力强的HLA-Ⅱ类等位基因;并根据该等位基因在亚洲不同地域的基因频率,预测H7N9的易感人群。结果:H7N9流感病毒株之间氨基酸序列相对保守;HA具有10个B细胞表位和15个T细胞表位;NA有12个B细胞表位和9个T细胞表位;HLA等位基因DRB1*0701与HA、NA具有强结合力,在新疆、哈尔滨、山东、辽宁、北京、石家庄、天津等多个中国北方地区人群中的基因频率较高,高于广东、云南、台湾地区、日本和韩国。结论:预测了HA、NA的抗原性表位,为H7N9流感病毒的疫苗研究提供了理论基础;HLA-DRB1*0701与H7N9流感病毒高度相关;H7N9流感病毒在新疆、哈尔滨、山东、辽宁、北京、石家庄、天津地区更易传播。Objective： To compare the amino acid sequences difference of HA,NA novel influenza virus A/H7N9 isolates,decipher possible B cell epitopes and T cell epitopes of HA,NA protein,and analyze the association between susceptibility and HLA polymorphisms. Methods： The amino acid sequences of novel influenza A（ H7N9） virus were downloaded from Genbank. Phylogenetic trees were constructed based on the amino acid sequences of HA and NA by using software Clustal X and MEGA 4. 0. B cell and T cell epitopes were respectively predicted with Protean software and Net MHCⅡ 2. 2 Server online server. Results： The homology of HA and NA proteins of H7N9 virus was high. 10 B cell epitopes and 15 T cell epitopes were randomly distributed throughout HA sequence and12 B cell epitopes and 9 T cell epitopes were randomly distributed throughout NA sequence. HLA-DRB1＊ 0701 allele which was commonly observed in Northern Chinese population have a high binding affinity for 9-mer peptides of HA and NA proteins. Conclusion：The prediction of B and T cell epitopes of HA and NA proteins with multiple methods benefits the research and development of vaccine against human infection with avian influenza A H7N9 virus. HLA-DRB1＊ 0701 allele may contribute to susceptibility to novel influenza A（ H7N9） virus. H7N9 influenza virus is more easily spread in Urumqi,Harbin,Shandong Province,Liaoning Province,Beijing,Shijiazhuang and Tianjin of China.

关 键 词：H7N9流感病毒 抗原性表位 HLA 易感性 血凝素 神经氨酸酶 

分 类 号：R392.8[医药卫生—免疫学]