Graves病患者^(131)I、甲巯咪唑治疗前后外周血Th17细胞及IL-17水平的变化  

Change of levels of peripheral Th17 cells,IL-17 with Graves' disease before and after ^(131)I,methimazol treatment

作　　者：郭泽华[1] 于世鹏[2] 班博[1] 

机构地区：[1]山东大学,济南250100 [2]济宁医学院附属医院,济宁272000

出　　处：《中国免疫学杂志》2015年第1期117-121,共5页Chinese Journal of Immunology

摘　　要：目的:通过比较研究GD患者131I、甲巯咪唑治疗外周血中Th17细胞、IL-17的变化,探讨Th17细胞在GD发病机制中的作用及意义。方法:收集初诊的GD患者31例采用131I治疗,30例采用甲巯咪唑(MMI)治疗,分别在治疗前(T0)及治疗后1个月(T1)、3个月(T3)进行相关指标测定。另选年龄、性别匹配的29例正常人作为正常对照组。应用流式细胞仪技术检测外周血中Th17细胞比例。采用ELISA法检测各组血清IL-17水平。结果:2种治疗方案T0组外周血Th17细胞及IL-17水平明显高于正常对照组(P<0.01)。131I治疗组外周血Th17细胞及IL-17水平在T0、T1、T3组中水平逐渐下降,仍高于正常对照组(P<0.01),MMI治疗组变化无统计学差异(P>0.05)。采用双变量相关性分析得出Th17细胞比例与IL-17水平呈正相关(r=0.758,P<0.05),且两者与甲状腺相关抗体呈正相关。结论:Th17细胞、IL-17在初发GD中高表达,Th17细胞可能参与了GD的发病过程。另外,放射性131I可能通过影响Th17细胞及IL-17起到治疗GD的作用。Objective： To explore the role of Th17 cells in the pathogenesis with Graves＇ disease（ GD）,through examining the proportion of peripheral of Th17 cells and the plasma levels of Interleukin-17（ IL-17） before and after131^ I or methimazol（ MMI） treatment with GD patients. Methods： We studied 31 subjects and 30 subjects with GD after131^ I or MMI treatment as131 I therapy group and MMI therapy group and examined as before treatment（ T0）,one month（ T1） and three months（ T3） after the treatment. 29 age- and sexmatched healthy subjects were enrolled as the healthy control group. The Th17 lymphocytes cells were investigated by flow cytometry analysis. The levels of serum IL-17 were measured by ELISA. Results： The proportion of peripheral Th17 cells and the levels of IL-17 were significantly increased in the T0 group GD patients compared with the control group（ P〈 0. 01）. In the131 I therapy group the levels of Th17 cells,IL-17 in T0,T1,T3 decreased gradually,but still higher than in normal group（ P 〈0. 01）. But there was no significant difference in MMI therapy group（ P 〈0. 05）. The proportion of peripheral Th17 cells had positive relationship with the level of IL-17（ r = 0. 758,P 0. 05）. Both of them were associated positively with thyroid autoantibodies. Conclusion： Th17 cells,IL-17 are highly expressed in the GD. Th17 cells may play an important role in the pathogenesis of GD. In addition,radioactive131 I may also work on GD via Th17 cells and IL-17.

关 键 词：GRAVES病 TH17细胞 IL-17 发病机制 131^I治疗 甲巯咪唑治疗 

分 类 号：R392.1[医药卫生—免疫学]