RP-HPLC法同时测定成人血清中苦参素和阿德福韦酯的浓度方法学研究  

Determination of oxymatrine and adefovir dipivoxil in human blood serum by RP-HPLC

作　　者：陈纭[1] 黄晓晖[1] 吴婷妮[1] 魏丽华[1] 杨海婷[1] 刘真真[1] 李冬[1] 李琳琳[1] 潘亭[1] 

机构地区：[1]安徽医科大学药学院,安徽合肥230032

出　　处：《安徽医药》2015年第1期58-60,共3页Anhui Medical and Pharmaceutical Journal

基　　金：国家自然科学基金项目(No 81173133);安徽医科大学七年制临床医学专业"早期接触科研"训练计划项目(No 2011-ZQKY-94)

摘　　要：目的：建立一种简易的测定联合服用苦参素和阿德福韦酯成人血清中苦参素和阿德福韦酯含量方法。方法采用Shim-pack VP-ODS C18色谱柱（250 mm×4．6 mm，5μm），流动相为甲醇∶水（70∶30），流速为1．1 mL·min －1，检测波长为229 nm。结果苦参素保留时间4．6 min 左右，标准曲线的线性范围为0．0125～2 mg·L －1，r ＝0．9992。阿德福韦酯保留时间7．3 min 左右，标准曲线的线性范围为0．0125～0．75 mg·L －1，r ＝0．9991，苦参素和阿德福韦酯的日间、日内变异系数均在7％以下，回收率＞92％。结论该法简便，灵敏，快速，可用于苦参素和阿德福韦酯临床联用治疗的成人血药浓度的监测。Objective To establish a method for determination of oxymatrine and adefovir dipivoxil in human serum.Methods RP-HPLC method was used,with C18 column at the standard of 250 mm ×4.6 mm,methanol-water（70∶30）as mobile phase.The flow rate was 1.1 mL·min -1 ,with the detection wavelength at 229 nm.Results The reserve time of oxymatrine in human blood serum was 4. 6 min.The linearity range of standard curve was 0.0125 ~2 mg·L -1 （r =0.999 2）.The reserve time of adefovir dipivoxil in human blood serum was 7.3 min.The linearity range of standard curve was 0.012 5 ~0.75 mg·L -1 （r =0.999 1）.The average recoveries of oxymatrine and adefovir dipivoxil were both above 92%.The intraday and extraday CVs were below 7%.Conclusions The method is simple,sensitive,rapid and can be used for monitoring and analysis of oxymatrine and adefovir dipivoxil in human blood serum.

关 键 词：苦参素 阿德福韦酯 反相高效液相色谱 血清浓度 

分 类 号：R96[医药卫生—药理学] O657.72[医药卫生—药学]