胃癌差异表达蛋白的蛋白质组学分析  

Proteomics Analysis of Differential Expression Proteins in Gastric Carcinoma

作　　者：李素云[1,2] 陈苏琼[1] 谢远杰[1] 张志伟[1] 贺修胜[1] 

机构地区：[1]南华大学肿瘤研究所,湖南衡阳421001 [2]南华大学医学院解剖教研室

出　　处：《中南医学科学杂志》2015年第1期14-20,共7页Medical Science Journal of Central South China

基　　金：湖南省自然科学基金资助项目(12JJ3102);湖南省卫生厅科研基金资助项目(B2012-046);衡阳市科技局资助项目(2013HKJ19);湖南省高校创新平台开放基金资助项目(13K082);地方高校国家级大学生创新创业训练计划资助项目(2012105555017)

摘　　要：目的筛选与鉴定胃癌差异表达蛋白质分子。方法采用定量蛋白组学方法,通过同位素i TRAQ标记胃癌与正常胃黏膜组织总蛋白,然后用强阳离子交换/反相液相色谱(SCX/nano HPLC)进行电喷雾串联质谱分析(ESI-MS/MS),获得两组样本的多肽及相对丰度信息,通过数据库搜索与比对,鉴定差异表达蛋白质。结果共鉴定出319个蛋白质,其中表达差异在2倍以上的88个,在胃癌中呈高表达的12个、呈低表达的76个。按蛋白质功能分为七大类:细胞骨架蛋白、分子伴侣、信号传导、生物代谢、凋亡,蛋白质合成与代谢类及其他蛋白质。结论成功筛选了胃癌差异表达蛋白质分子,这些蛋白质的表达改变,可能参与了胃癌的发生和发展。Objective To identify the differential expression proteins between gastric adenocarcinoma cells（ GAC）and normal gastric adenoepithelial cells（ NGAC）,and to look for early diagnosis of Gastric carcinoma（ GC） specific proteins. Methods The total proteins were extracted from the tissues. The total proteins labeled with different i TRAQ stable isotope were segregated with Nano-RPLC-MS / MS,to obtain relative abundance of peptides information. The differential expression proteins were identified through SWISSPORT database. Results Through Quantitative proteomic analysis of GAC and NGAC,319 proteins were identified with MS,88 of which were differential expression proteins. There were 12 Upregulation and 76 Down-regulation proteins in GAC. The identified proteins can be divided into seven groups according to protein function： biological oxidation,signal transduction,protein composition and metabolism,molecular chaperones,cytoskeleton,apoptotic and others. Conclusion The differentially expressed protein molecules were successfully screened and identified in gastric carcinoma,and the expression changing of these proteins may be involved in the genesis and development of gastric carcinoma.

关 键 词：胃癌 iTRAQTM同位素标记相对和绝对定量 多维液相色谱 串联质谱 

分 类 号：R735.2[医药卫生—肿瘤]