荠菜多糖的分离纯化与单糖组成分析  

Isolation,Purification and Monosaccharide Composition Analysis of Polysaccharide from Shepherdspurse Herb

作　　者：王华[1] 熊淑娟[2] 曹轩[2] 陈临溪[2] 唐国涛[2] 

机构地区：[1]南华大学附属第一医院药剂科,湖南衡阳421001 [2]南华大学药物药理研究所

出　　处：《中南医学科学杂志》2015年第1期82-85,共4页Medical Science Journal of Central South China

基　　金：湖南省中医药科研计划项目(201162)

摘　　要：目的对荠菜多糖进行分离纯化并分析其单糖组成。方法采用超声法提取总多糖,除蛋白,用Sephadex G-75进行纯化,水解后用气相色谱分析其单糖组成。结果分离得到两个多糖组分1和2。气相色谱鉴定出其中四种单糖组成,D-木糖、L-鼠李糖、D-甘露醇和D-葡萄糖,其组成比例为1:1.8:270.8:1。结论从荠菜多糖中分离出两种多糖组分,组分2由8种多糖组成,鉴定出其中4种。Objective to isolate and purify the polysaccharide and analyze the monosaccharide composition. Methods the polysaccharide extraction removing the proteins were purified through sephadex G75,the polysaccharide components were analyzed by gas chromatography（ GC） after hydrolization. Relsults two monosaccharide components were obtained after purification. Four monosaccharide compositions concluding D-xylose,L-rhamnose,D-mannose and D-glucose were measured by GC in the second components and the proportion were 1∶1. 8∶ 270. 8∶ 1. Conclusion two polysaccharide fractions were isolated from Shepherdspurse Herb. The second fraction was composed of eight kinds of monosaccharide and four of them were identified.

关 键 词：荠菜多糖 分离纯化 单糖组成 气相色谱 

分 类 号：R917[医药卫生—药物分析学]