星形胶质细胞在大鼠选择性结扎镜像痛模型脊髓中的激活和意义  

Activation and Significance of Astrocytes in Spinal Cord of Spinal Nerve Selective Ligation Rats' MirrorImage Pain Model

作　　者：王江栓[1] 李变锋[1] 任秀华[2] 曹靖[2] 臧卫东[2] 田志逢[1] 

机构地区：[1]漯河医学高等专科学校,河南漯河462000 [2]郑州大学

出　　处：《中国医学创新》2015年第3期18-20,共3页Medical Innovation of China

基　　金：河南省2013年科技发展计划项目(132102310358);漯河医学高等专科学校科学研究计划项目(2013-S-LMC25)

摘　　要：目的：观察星形胶质细胞在大鼠选择性结扎镜像痛模型脊髓中的激活情况。方法：实验组选择性结扎（SNL）镜像痛模型大鼠20只,对照组SNL非镜像痛模型大鼠20只,取第4-5腰椎段脊髓做石蜡切片,免疫荧光组织化学标记胶质纤维酸性蛋白（GFAP）的表达。结果：实验组非手术侧（镜像痛）脊髓背角GFAP免疫阳性细胞数量增多,平均荧光强度增高,与手术侧比较差异无统计学意义（P〉0.05）;但与对照组非手术侧比较差异有统计学意义（P〈0.05）。结论：在神经病理性疼痛镜像痛中,镜像侧脊髓背角星形胶质细胞活化并参与了镜像痛的形成和维持。Objective：To investigate the activation of astrocytes in spinal cord of spinal nerve selective ligation（SNL） rats＇ mirror-image pain model. Method：The experimental group（n=20） was SNL mirror-image pain model group, the control group（n=20） was SNL model group. Every group was took the L4-L5 spinal cord to make paraffin slices. Labeled the expression of glial fibrillary acidic protein（GFAP） by immunofluorescence method.Result：The positive cells GFAP marked were increased in the number and the mean fluorescence intensity in the experimental groups＇ spinal dorsal horn of non-surgical side which had no statistical significance（P〈0.05） compared with the surgical side, but had statistical significance（P〈0.05） compared with the non-surgical side of the control group. Conclusion：In the neuropathic mirror-image pain, astrocytes were activated and involved the formation and maintenance of mirror pain in the mirror side of the spinal cord.

关 键 词：星形胶质细胞 神经病理性疼痛 镜像痛 

分 类 号：R651.2[医药卫生—外科学]