卡介菌多糖核酸对慢性阻塞性肺疾病T淋巴细胞核仁区酸性非组蛋白水平的影响  

Effects of BCG-polysaccharide Nucleic Acid on T-lymphocytes Argyrophilic Nucleolar Organizer Proteins in COPD Patients

作　　者：唐孝庆[1] 张程[1] 叶贤伟[1] 刘维佳[1] 张湘燕[1] 

机构地区：[1]贵州省人民医院呼吸与危重症医学科,贵州省贵阳市550002

出　　处：《中国全科医学》2015年第2期172-175,共4页Chinese General Practice

基　　金：贵州省发展和改革委员会资助项目(黔发改高技[2006]858号)

摘　　要：目的探讨卡介菌多糖核酸对慢性阻塞性肺疾病(COPD)患者T淋巴细胞核仁区酸性非组蛋白(Ag-NORs)水平的影响。方法选择2012年1月—2013年6月贵州省人民医院呼吸与危重症医学科及干部医疗科收治的COPD急性加重期(AECOPD)患者50例及在两科室治疗后COPD稳定期患者40例。采用随机数字表法将AECOPD患者和COPD稳定期患者分别分为治疗组和对照组。AECOPD及COPD稳定期对照组患者均仅给予常规治疗,治疗组患者在常规治疗基础上加用卡介菌多糖核酸治疗,疗程均为1个月。于治疗前后均对患者外周血T淋巴细胞Ag-NORs进行染色分析,以核仁银染面积/核银染面积(I/S%)作为Ag-NORs的检测指标。结果治疗前,AECOPD治疗组和AECOPD对照组外周血T淋巴细胞Ag-NORs水平比较,差异无统计学意义(P>0.05);治疗后,AECOPD治疗组外周血T淋巴细胞Ag-NORs水平高于AECOPD对照组(t=-1.708,P<0.05)。治疗前,COPD稳定期治疗组和COPD稳定期对照组外周血T淋巴细胞Ag-NORs水平比较,差异无统计学意义(P>0.05);治疗后,COPD稳定期治疗组外周血T淋巴细胞Ag-NORs水平高于COPD稳定期对照组(t=-1.647,P<0.05)。AECOPD治疗组及COPD稳定期治疗组治疗期间各出现注射部位局部红肿1例,予热敷后1周内自然消退,其他均未出现明显不良反应。结论 COPD患者使用卡介菌多糖核酸治疗,可增加T淋巴细胞Ag-NORs水平,改善其免疫功能。Objective To detect the effects of BCG - polysaccharide nucleic acid on peripheral blood T - lymphocytes argyrophilic nucleolar organizer proteins （ Ag - NORs） in patients with chronic obstructive pulmonary disease （ COPD）. Methods Fifty patients of acute exacerbation of COPD（ AECOPD） were divided randomly into groups AECOPD study, AECOPD control and 40 remitting COPD（RCOPD）patients into groups RCOPD study,RCOPD control in Peopleˊs Hospital of Guizhou from january 2012 to june 2013. Four groups were given conventional treatment,the study groups given additional BCG- polysaccharide nucleic acid,the courses lasted 1 month. Taking nucleolar silver staining/ nuclear silver staining（I/ S% ）as the test index of Ag - NORs,a staining analysis on peripheral blood T - lymphocyte Ag - NORs was performed before and after treatment. Results There was no significant difference in Ag - NORs level between groups AECOPD study,control before treatment（P 〉 0. 05）,higher in AECOPD study group than in AECOPD control after treatment（t = - 1. 708,P 〈 0. 05）. No difference was noted in Ag - NORs between groups RCOPD study,control before treatment（P 〉 0. 05）,and higher in RCOPD study group than in RCOPD control group after treatment（t = - 1. 647,P 〈 0. 05）. One patient from AECOPD study group and 1 from RCOPD study group had local swelling on injection sites and regressed spontaneously. No obvious adverse reactions appeared in the other patients. Conclusion BCG - polysaccharide nucleic acid can increase T - lymphocyte Ag - NORs level and improve its immune function in COPD patients.

关 键 词：肺疾病 慢性阻塞性 卡介菌多糖核酸 核仁区酸性非组蛋白 T淋巴细胞 免疫 

分 类 号：R563[医药卫生—呼吸系统]