熊果酸对顺铂致毒小鼠耳蜗瞬时感受器电位香草酸受体1表达的影响  

The Effects of Ursolic Acid on Cisplatin-Induced Expression of Transient Receptor Potential Vanilloid 1 in Mouse Cochlea

作　　者：邸阳[1] 于利[1] 田原[1] 林宇涵[1] 王爱梅[1] 

机构地区：[1]辽宁医学院生理学教研室,锦州121001

出　　处：《听力学及言语疾病杂志》2015年第1期57-60,共4页Journal of Audiology and Speech Pathology

基　　金：辽宁省科学技术计划项目(2011225015)

摘　　要：目的研究熊果酸(ursolic acid,UA)对顺铂(cisplatin,CDDP)致毒小鼠耳蜗瞬时感受器电位香草酸受体1(transient receptor potential vanilloid 1,TRPV1)表达的影响。方法 60只健康BALB/c小鼠随机分成对照组(腹腔注射等量生理盐水)、UA组(腹腔注射熊果酸80mg/kg)、CDDP组(腹腔注射CDDP 4.5mg/kg)和CDDP+UA组(一侧腹腔注CDDP 4.5mg/kg,同时对侧腹腔注射熊果酸80mg/kg),每组15只。各组动物均连续腹腔注射给药5天,每天1次,给药前及停药后24h行ABR检测,然后应用免疫组织化学染色、显微图像分析及蛋白质印迹技术观察小鼠耳蜗中TRPV1的表达。结果 CDDP组小鼠耳蜗TRPV1表达和ABR阈移均较对照组明显增加(P<0.05),UA+CDDP组小鼠ABR阈移及TRPV1表达较CDDP组明显降低(P<0.05),且TRPV1表达变化与ABR阈移改变高度相关(|r|>0.7,P<0.05)。结论 UA可抑制CDDP所致TRPV1的高表达,有效降低CDDP的耳毒性,改善听功能。Objective To investigate the effects of ursolic acid （UA） on cisplatin （CDDP）-induced expres_sion of transient receptor potential vanilloid 1 （TRPV1） in mouse cochlea .Methods Sixty BALB/c mice were ran_domly divided into 4 groups （15 mice in each group） and received introperitoneal injection once daily for 5 days：Control group （normol saline） ,UA group （80 mg/kg/day） ,CDDP group （4 .5 mg/kg/day） ,and CDPP （4 .5 mg/kg/day） plus UA group （80 mg/kg/day） .The expression of TRPV1 in mouse cochlea was determined by immuno_histochemistry ,microscope image analysis and western blot ,and auditory thresholds were evaluated by auditory brainstem response （ABR） measurement .ResuIts The expression of cochlea TRPV1 and ABR threshold shift was significantly increased in the mice treated with CDDP （P〈 0 .05） ,as compared with control mice .These effects were prevented by UA treatment （all P〈0 .05） .Furthermore ,a linear relationship analysis revealed that the ex_pression of cochlea TRPV1 was significantly correlated with ABR threshold shift（｜r｜〉0 .7 , P〈0 .05） .ConcIusion UA effectively attenuated CDDP -induced ototoxicity and improved auditory function through inhibition of TR_PV1 .

关 键 词：熊果酸 顺铂 耳蜗 瞬时感受器电位香草酸受体1 

分 类 号：R764.43[医药卫生—耳鼻咽喉科]