组织培养结合秋水仙素诱导南瓜多倍体  

Study on polyploid induction of pumpkin by tissue culture combined with colchicines

作　　者：闵子扬[1] 童龙[1] 杨红波[1,2] 李涵[1] 成娟[1] 张琼[1,2] 谢向阳[2] 孙小武[1,2] 

机构地区：[1]湖南农业大学,长沙410128 [2]湖南省瓜类研究所,湖南邵阳422001

出　　处：《中国瓜菜》2015年第1期15-18,共4页China Cucurbits And Vegetables

基　　金：国家现代农业产业技术体系建设专项(CARS-26-09)

摘　　要：以‘红栗2号’南瓜子叶为外植体,探讨外植体消毒时间、激素组合和培养基中秋水仙素浓度对芽诱导率的影响,对所得植株的倍性进行鉴定。结果表明:外植体在3%(w)Na Cl O溶液中消毒15 min,污染率为0且外植体活力较高;MS+6-BA 3.0 mg·L-1+NAA 0.1 mg·L-1+秋水仙素40 mg·L-1是诱导‘红栗2号’南瓜产生四倍体的最适培养基,诱导率达到25.71%;组培苗形态及根尖染色体有四倍体存在。The cotyledon of pumpkin-＇ Hongli 2＇ is used as explant to study the influence of disinfect time, hormone combi- nation and different concentrations of colchicine of medium on bud induction rate. The ploidy of regenerated plant is also identified .The results showed that the explants disinfect in 3% Naelo solution for 15 rain, the pollution rate can be reduced to zero and the explants showed higher activity. MS＋6-BA 3.0 mg/L＋NAA 0.1 mg/L＋eolchicine 40 mg/L was the optimal me- dium to induce ＇Hongli 2＇ to generate tetraploid. The bud induction rate was up to 25.71%. The observation of somaclone form and root tip chromosomes showed the existence of tetraoloid.

关 键 词：南瓜 '红栗2号’ 组织培养 秋水仙素 诱导 四倍体 

分 类 号：S642.1[农业科学—蔬菜学]