电针足三里穴对失血性休克大鼠肠胶质细胞的影响  

Effects of electro-acupuncture of Zusanli point(足三里穴) on the enteric glia cells in rat with hemorrhagic shock

作　　者：赵增凯[1,2] 石现[3] 胡森[2] 王欢[1,2] 罗红敏[1,2] 

机构地区：[1]解放军医学院,北京100853 [2]解放军总医院第一附属医院烧伤研究所休克与多器官障碍实验室,北京100048 [3]解放军总医院针灸科,北京100853

出　　处：《感染．炎症．修复》2014年第3期136-139,F0003,共5页Infection Inflammation Repair

基　　金：国家重点基础研究发展计划(973)项目(2012CB518101);全军医学科研"十一五"计划项目(06Z055)

摘　　要：目的：观察电针足三里穴对失血性休克大鼠肠胶质细胞形态的影响,探讨电针足三里穴治疗胃肠疾病的机制。方法：将18只SD雄性大鼠随机分为假手术组、模型组和电针组,每组6只。按总血容量的45%放血制备失血性休克模型。电针组于制模完成后10 min电针足三里穴（持续刺激1.5 h,2-100 Hz,2 mA）;模型组同样方法电针非经非穴;假手术组只分离股动、静脉不放血。失血后6 h后处死大鼠,取肠道组织制作肠肌间神经丛全层铺片,采用免疫荧光法进行肠胶质纤维酸性蛋白（GFAP）染色,荧光显微镜下观察肠胶质细胞形态,同时对肠道GFAP蛋白含量进行WesternBlot测定。结果：失血后6 h,肠胶质细胞出现形态学异常,细胞变形并且荧光增强,突触模糊、中断;电针足三里穴能减轻肠胶质细胞形态学异常,增加荧光强度。与假手术组相比,模型组GFAP表达量增加,但电针足三里穴后GFAP的表达量增加更为显著（P〈0.05）。结论：大鼠失血性休克后电针足三里穴能激活并保护肠胶质细胞。Objective：To investigate the effects of electro-acupuncture of Zusanli point（足三里穴） on enteric glial cells of the rats after hemorrhagic shock,and inquire into the mechanism of electro-acupuncture of Zusanli points in treating gastrointestinal disease.Methods：Eighteen male SD rats were randomly divided into three groups（n = 6）：sham operation group,model group,and electro-acupuncture group.Hemorrhagic shock was replicated by withdrawing 45%of the calculated total blood volume.Rats in electro-acupuncture group received electro-acupuncture at Zusanli points（ST36） 10 minutes after blood loss（electro-acupunctured for 1.5 hours,2-100 Hz,2 mA）.Electro-acupuncture at non-channel acupoints was performed in rats of model group.Rats in sham operation group did not suffer from blood loss.Six hours after hemorrhage,animals were sacrificed,and distal small intestine was harvested to prepare whole mount slide of myenteric plexuses.Morphological changes in enteric glial cells（EGCs） were observed with immunofluorescence staining of glial fibrillary acidic protein（GFAP）,and the quantity of GFAP was also determined by Western blotting.Results：Six hours after hemorrhage,morphological abnormalities including disfigurement of EGCs with stronger fluorescence were observed.The synapses of EGCs were distorted and became blurred or even discontinued.Electro-acupuncture at ST36 was found to attenuate morphological changes in EGCs,and enhance the fluorescence.Comparing with the group of sham operation,the expression of GFAP was enhanced in rats of model group,while GFAP expression was even significantly higher after electro-acupuncture at ST36（P〈0.05）.Conclusions：Electroacupuncture at ST36 can activate and protect the EGCs in rats with hemorrhagic shock.

关 键 词：休克 失血性 足三里穴 电针 肠胶质细胞 全层铺片 大鼠 

分 类 号：R605.971[医药卫生—急诊医学]