血催化鲁米诺化学发光性能的研究  

Study on Chemiluminescence Property of Luminol Catalyzed by Blood

作　　者：蔡俊[1] 伍斌[1] 邓鸾姣 冯志明[1] 

机构地区：[1]湖南师范大学化学生物学及中药分析省部共建教育部重点实验室,湖南长沙410081

出　　处：《化学与生物工程》2014年第12期42-43,66,共3页Chemistry & Bioengineering

摘　　要：采用荧光分光光度法,建立了血催化过氧化氢氧化鲁米诺的化学发光体系。考察了缓冲体系、pH值、血液浓度等对鲁米诺化学发光性能的影响。结果表明,当采用56.60mmol·L-1 Na2CO3-NaHCO3缓冲体系与30.0mmol·L-1 H2O2溶液(B液)混合时,在pH=8.98的条件下,血液浓度越高,血催化鲁米诺化学发光性能越好。Chemiluminescence system of hydrogen peroxide oxidation of luminol catalyzed by blood was establised by fluorescence spectrophotometry.The effect of buffer system,pH value and blood concentration on the chemiluminescence property of luminol were studied.Results showed that,when using 56.60 mmol·L^-1Na2CO3-NaHCO3 as buffer system,mixing with 30.0 mmol·L^-1 H2O2（solution B）,pH value was 8.98,the higher the concentration of blood,the luminol chemiluminescence property catalyzed by blood was better.

关 键 词：荧光 鲁米诺 化学发光 性能 

分 类 号：O657.1[理学—分析化学]