西兰花种子中萝卜硫素的提取工艺研究  

Study on Extraction Process of Sulforaphane from Broccoli Seeds

作　　者：林毅[1] 张金娟[1] 李晓露[1] 李宁[1] 王海燕[1] 任凤芝[1] 马志珺[1] 

机构地区：[1]微生物药物国家工程研究中心,河北省工业微生物代谢工程技术研究中心,华北制药集团新药研究开发有限责任公司,河北石家庄050015

出　　处：《化学与生物工程》2014年第12期48-50,共3页Chemistry & Bioengineering

摘　　要：以西兰花种子为原料,提取其中的萝卜硫素,考察了水解pH值、水解时间、萃取溶剂对提取率的影响。结果表明,在水解pH值为4.0、水解时间为12h、萃取溶剂为乙酸乙酯时,萝卜硫素提取率达3.5%,提取纯度超过95%。Extraction of sulforaphane from broccoli seeds was studied in this paper.The effects of hydrolysis pH value,hydrolysis time,extraction solvent on extraction rate of sulforaphane were investigated.Results showed that when hydrolysis pH value was 4.0,hydrolysis time was 12 h,extraction solvent was ethyl acetate,the extraction rate of sulforaphane reached 3.5% and the purity was up to 95%.

关 键 词：萝卜硫素 西兰花种子 水解 分离 纯化 固相萃取 

分 类 号：TQ028[化学工程] O623.8[理学—有机化学]