产妇会阴切口感染肺炎克雷伯菌质粒介导AmpC酶基因型检测与分析  

Genetic detection of plasmid-mediated AmpC beta-lactamase in Klebsiella pneumoniae from perineal incision infection

作　　者：金海萍[1] 杨慧雅[1] 曹海华[1] 边保华[2] 

机构地区：[1]台州医院产科,浙江临海317000 [2]台州学院医学院检验系,浙江台州317000

出　　处：《中国微生态学杂志》2014年第12期1449-1451,共3页Chinese Journal of Microecology

摘　　要：目的回顾性分析我院产妇会阴切口感染分离的肺炎克雷伯菌质粒介导AmpC酶基因型。方法采用K-B纸片扩散法对临床分离的肺炎克雷伯菌进行AmpC酶初筛;头孢西丁三维试验确证产AmpC酶;采用UNIQ-10柱式质粒小量抽提试剂盒提取细菌质粒;采用聚合酶链反应(PCR)和DNA测序检测分析质粒AmpC酶基因型别。结果三维试验检出15株产AmpC酶菌株,经PCR扩增15株菌株均在405bp处出现阳性条带,经DNA测序证实为DHA-1型质粒AmpC酶。结论我院存在质粒介导AmpC酶流行菌株,质粒AmpC酶主要以DHA-1型为主。Objective To retrospectively analyze the genotype of plasmid-mediated AmpC in Klebsiella pneumoniae isolated from patients with perineal incision infection in our hospital. Methods Kirby-Bauer agar disk diffusion method was used to screen the AmpC enzyme from the clinical isolates of Klebsiella pneumoniae ; Three-diMensional test with cefoxitin were used to confim the production of AmpC enzyme. Extraction of bacterial plasmids was conducted by using the UNIQ-IO column plasmid minipreps Kit. Polymerase chain reaction （PCR） and DNA sequence analysis were performed to detect the genotype of plasmid AmpC enzyme. Results Total 15 positive strains were detected by three dimensional test, all of which showed positive 405 bp bands, and PCR sequencing confirmed that the 15 strains carried DHA-1 type AmpC gene. Conclusion Plasmid-mediated AmpC enzyme-producing strains exist in our hospital, with DHA-1 being the main type

关 键 词：质粒 AMPC酶 基因型 肺炎克雷伯菌 产妇 

分 类 号：R379[医药卫生—病原生物学]