烧伤愈合膏中7种成分的含量测定  

Determination of seven components in SSYHG

作　　者：章道华[1] 徐彬[2,3] 吴金虎 陈永刚 邹吉利 

机构地区：[1]华中科技大学同济医学院附属协和医院西院药剂科,湖北武汉430056 [2]湖北中医药大学药学院,湖北武汉430065 [3]湖北随州市中心医院药学部,湖北随州441300 [4]武汉市第三医院药学部,湖北武汉430060

出　　处：《中国医院药学杂志》2014年第24期2099-2102,共4页Chinese Journal of Hospital Pharmacy

基　　金：湖北省科技计划自然科学基金(编号:2011CDB298);武汉市人力资源与社会保障局关键技术攻关计划项目(编号:2013060602010264)

摘　　要：目的：建立同时测定烧伤愈合膏中盐酸黄连碱、盐酸巴马汀和盐酸小檗碱含量的液相色谱测定法,并建立同时测定其中α-蒎烯、柠檬烯、异龙脑和龙脑的气相色谱测定法。方法：高效液相色谱法采用Genimi-C18柱,以乙腈-0.04 mo L·L^-1磷酸二氢钠溶液（30∶70）为流动相,检测波长：345 nm;气相色谱法采用DB-WAX毛细管柱（30 m×0.25 mm,0.25μm）,程序升温80℃（5 min）→5℃·min^-1→180℃（10 min）,分流进样,分流比为10∶1;载气为氮气,氢火焰离子化检测器。结果：盐酸黄连碱、盐酸巴马汀、盐酸小檗碱、α-蒎烯、柠檬烯、异龙脑和龙脑在各自浓度范围内呈良好线性关系;平均回收率分别在96%-102%之间。结论：所建立的2种测量方法简单、精确、可行,可作为烧伤愈合膏质量控制的标准,为其再次研发奠定了基础。OBJECTIVE To establish a method for determination of components（ Coptisine hydrochloride、Palmatine chloride、Berberine Hydrochloride、α-pinene、limonene、isoborneol and borneol） in SSYHG. METHODS There components were determined by HPLC,the column was Genimi-C18 column,using acetonitrile（ A）- 0. 04 mo L·L^- 1sodium dihydrgenj phosphate（ B） as mobile phase,wavelength was 345 nm. Four volatile components were determined on DB-WAX capillary column（ 30 m × 0. 25 mm,0. 25 μm）by GC. The column temperature was raised by program,80 ℃（ 5 min） →5 ℃·min^- 1→180 ℃（ 10 min）; Split stream sampling was applied,split ratio was 10∶ 1; carrier gas was nitrogen; detector： FID. RESULTS The seven components were isolated well. The linearities were fine with the recoveries of 96%- 102%,respectively. CONCLUSION The two methods are convenient、rapid and accurate,they can be used to control the quality of SSYHG.

关 键 词：烧伤愈合膏 高效液相色谱法 气相色谱法 含量测定 

分 类 号：R927[医药卫生—药学]