天然牛磺酸对肝硬化大鼠MMP-9、Integrin-β1表达的调控  

Regulation Effect of Natural Taurine On MMP-9,Integrin-β1 Expression In Rats With Liver Cirrhosis

作　　者：文彬[1] 陈然[1] 彭佩纯 邓鑫[1] 

机构地区：[1]广西中医药大学附属瑞康医院.南宁530011

出　　处：《天然产物研究与开发》2014年第12期1939-1943,共5页Natural Product Research and Development

基　　金：国家自然科学基金(81160433);广西科技厅重点课题(2011GXNSFD018035);广西卫生厅重点课题(重20122031);中南大学博士后科学基金(2013M531816)

摘　　要：天然牛磺酸广泛存在于海洋生物中,研究发现具有一定抗纤维化作用,基质金属蛋白酶(MMP-9)、整合素β1(Integrin-β1)与肝纤维化密切相关,实验中通过给予肝硬化大鼠不同剂量天然牛磺酸(0.3、0.6、1.2 g/kg·d),采用放射免疫法检测血清肝纤四项含量;实时荧光定量PCR检测大鼠肝组织MMP-9、Integrin-β1 mRNA表达,免疫蛋白印迹法(Western-blot)检测相应蛋白的表达。结果表明,肝硬化大鼠MMP-9、Integrin-β1 mRNA及蛋白表达显著升高,天然牛磺酸能显著减少模型大鼠血清肝纤四项水平,降低MMP-9、Integrin-β1 mRNA及相关蛋白表达,以0.6 g/kg·d剂量组效果最佳。天然牛磺酸下调肝硬化大鼠MMP-9和Integrin-β1的表达,与其发挥肝保护作用密切相关。Natural taurine widely exists in marine organisms,and it has been reported to exert certain anti-fibrosis activity. Matrix metalloproteinase（ MMP-9）,Integrin β1 is closely related to liver fibrosis. In this study,liver cirrhosis rats were treated withdifferent doses of natural taurine（ 0. 3,0. 6,1. 2 g / kg·d）. Fourindices of serum liver fibrosis were analyzed by radioimmunoassay method. MMP-9,Integrin-β1 mRNA expression levels were detected in liver tissue through real-time PCR and related protein levels were determined by Western-blot analysis. The results showed that natural taurine can significantly reduce four indices of serum liver fibrosis in cirrhotic rats. In addition,natural taurinemarkedly reverted the elevated levels of MMP-9,integrin-β1 mRNA and related protein. The 0. 6 g / kg·ddosage group showed the highest activity. These results indicated that natural taurine downregulating of MMP-9 and Integrin-β1 expression was closely associated with its protective effects against liver cirrhosis in rats.

关 键 词：肝硬化 天然牛磺酸 基质金属蛋白酶 整合素-β1 细胞外基质 

分 类 号：R34[医药卫生—基础医学]