长尾草金鱼成熟期雌雄性腺RNA-Seq转录组分析  

The RNA-Seq Transcriptome Analysis in Mature Gonads of Long-tailed Goldfish Carassius auratus

作　　者：张升利[1] 付成东[2] 梁拥军[1] 李文通[1] 孙砚胜[1] 史东杰[1] 张欣[1] 

机构地区：[1]北京市水产科学研究所,农业部都市农业(北方)重点实验室,北京100068 [2]辽宁省海洋水产科学研究院,辽宁大连116023

出　　处：《水产科学》2014年第12期750-756,共7页Fisheries Science

基　　金：国家科技基础条件平台建设运行项目(2014DKA30470);北京市自然科学基金资助项目(6112008);现代农业产业技术体系北京市观赏鱼创新团队建设项目

摘　　要：为探究长尾草金鱼成熟期雌雄性腺的RNA-Seq转录组,运用Illumina RNA测序技术,对长尾草金鱼成熟期性腺分别进行了转录组测序。其中卵巢经两端测序共获得清洁数据61769892条,含有6.18G碱基,平均错误率0.03%,左右端测序GC含量分别为51.15%和51.2%。精巢经两端测序共测得清洁数据69557514条,含有6.96G碱基,平均错误率0.03%,左右端测序GC含量分别为48.91%和49.44%。两个转录组数据混合拼接后得到170245个转录本,共80591个独立基因。将上述独立基因与多个数据库进行比对,得到注释的基因为51364条,占总数的63.73%。基因本体分类中,细胞过程、细胞、绑定等二级分类为条数较多分类项。转录组数据经筛查共得到17347个微卫星标记和363416个单核苷酸多态性标记。两个转录组基因表达量差异比较结果显示,雌雄样本间表达差异基因共6317个。上述工作不仅为该种的基因克隆和标准及筛选提供了基础数据,也为分子水平阐明其性腺分化和发育机理打下了基础。The transcriptome of mature gonads was constructed and sequenced in long--tailed gold strain of goldfish Carassius auratus by Lllumina RNA sequencing. Through sequencing, 61769892 clean data in- cluding 6.18G bp were obtained in the ovary with an average error rate of 0.03%, and 69557514 clean data including 6.96G bp in testis with an average error rate 0.03%. The terminal GC content for library was 51. 15% in the left ovary and 51.2% in the right ovary, and 48.91M in the left testis and 49.44% in the right testis. The two libraries represented a total of 170245 transcripts, with 80591 unigenes. As deter- mined by the multiple databases, functional annotation of 51364 unigenes was obtained, accounting for 63.73 ~ of the total. All of the unigenes were found to be functional by classification of GO software, and unigenes involved in cell process, cell and binding occupied major proportion in the secondary classifica- tion. There were 17347 SSR markers and 363416 SNP markers throughout the transcriptomic data. The gene expression differences between the two transcriptome revealed that 6317 genes expressed differentially in male and female samples. The findings provided basic data for the gene cloning and screening of the goldfish, as well laid the foundation for clarifying the molecular mechanism of gonadal differentiation and development.

关 键 词：金鱼 性腺 转录组 微卫星 单核苷酸多态性 

分 类 号：S917.4[农业科学—水产科学]