儿童慢性特发性血小板减少性紫癜与HPA基因多态性的相关性研究  

Analysis on the relationship between chronic idiopathic thrombocytopenic purpura in children and genetic polymorphism of human platelet antigen systems

作　　者：唐利娟[1] 罗建明[1] 黄小花[1] 欧丹艳[1] 宾琼[1] 韦清华[1] 

机构地区：[1]广西医科大学第一附属医院儿科,广西南宁530021

出　　处：《中国输血杂志》2014年第12期1309-1312,共4页Chinese Journal of Blood Transfusion

摘　　要：目的了解HPA-1～6、15系统基因多态性在广西儿童慢性特发性血小板减少性紫癜（ITP）分布情况,探讨儿童慢性ITP与HPA-1～6、15系统等位基因的相关性。方法采用聚合酶链反应技术（PCR）结合直接测序方法,对儿童慢性ITP患者46例（ITP组）和正常健康儿童48例（对照组）进行HPA-1～6、15共7个抗原系统基因分型。结果 ITP组HPA-2、15系统基因频率明显异于对照组（P〈0.05）,其中HPA-2a及HPA-15a基因频率低于对照组,HPA-2b及HPA-15b基因频率高于对照组,其余系统等位基因频率在2组间的未发现有明显差别（P〉0.05）。结论HPA-2、15抗原系统多态性可能与儿童慢性ITP的易感性有关。Objectives To study the distribution of genetic polymorphisms of human platelet antigen HPA-1 - 6,15 sys- tems among Guangxi pediatric patients with chronic idiopathic thrombocytopenic purpura（CITP） and to investigate the rela- tionship between CITP and the genetic polymorphism of HPA-1 - 6,15 systems. Methods The genotypes of HPA-1 - 6 and 15 in the ITP group （n = 46） and the healthy control group （n = 48） were identified using polymerase chain reaction （PCR） combined with direct sequencing. Results The allele frequencies of HPA-2, 15 were significantly different between the ITP group and the control group （P =0. 004）. The frequency of HPA-2a, 15a in ITP group was lower than the control group. The frequency of HPA-2b, 15b was higher than the control group. There were no significant statistical differences between the frequencies of other alleles （ P 〉 0. 05 ）. Conclusion This study shows that genetic polymorphism in HPA-2, 15 antigen system may increase susceptibility to chronic ITP in children.

关 键 词：特发性血小板减少性紫癜 HPA 基因多态性 儿童 

分 类 号：R554.6[医药卫生—血液循环系统疾病] R457.11[医药卫生—内科学]