产妇ABO血型抗体效价不同检测方法的比较  

Comparison of the detection methods to measure the titer of antibody in maternal ABO blood

作　　者：张婵[1] 梁格为 李琦[2] 陈婉璐[2] 程瑜静[2] 彭慧玲[2] 

机构地区：[1]云南省第一人民医院输血科,云南昆明650031 [2]云南昆明血液中心

出　　处：《中国输血杂志》2014年第12期1349-1351,共3页Chinese Journal of Blood Transfusion

摘　　要：目的比较传统试管法与2种抗球蛋白检测卡,检测Ig G抗-A、抗-B效价水平的方法学差异与结果差异的相关性。方法对需要检测ABO血型抗体效价的920例O型产妇的送检血标本,采用试管法、微柱凝胶卡式法及惰性玻璃珠介质柱凝卡式法,对检测结果作比较分析。结果以效价为256做为临床参考界值,惰性玻璃珠介质柱凝卡式法阳性的人数共有261人;以128为临床参考界值,微柱凝胶卡式法阳性的人数共有265人;以64为界,试管法大于临床参考值的人数共有248人。结论卡式法比试管法具有灵敏度高,结果易于判读、保存的优势。但高灵敏度并不意味着高的HDN判断率,检测结果应与临床指征综合分析,才能有效的减少和杜绝HDN的误诊。Objectives To compare the conventional tube method and two types of antiglobulin test card methods on their ability to measure the titer of the antibody in maternal ABO blood and to investigate the relevance of the methods with the differences in results. Methods The titers of antibody in ABO blood in 920 cases of type O blood specimens ~atibody were measured using conventional tube method, micro-column gel cards and inert glass bead column gel cards. A compara- tive analysis was made on the test results. Results Using the titer of 256 as a clinical reference value, the numbers of peo- ple who were tested positive by inert glass bead column chromatography were 261 ; using the titer of 128, the numbers of pos- itives tested by micro-column gel chromatography were 488 ; using the titer of 64, the numbers of positives tested by the con- ventional tube method were 248. Conclusion The card tests were more sensitive than the conventional tube methods, easi- er to read, and have a higher advantage in preservation. However, high sensitivity does not signify high diagnosis rate of ／ HDN. A comprehensive analysis of test results and clinical indications is necessary to effectively reduce the prevalence of and prevent the misdiagnosis of HDN.

关 键 词：ABO血型 抗体效价 抗球蛋白 新生儿溶血病 

分 类 号：R446[医药卫生—诊断学]