深圳地区乙型肝炎病毒基因分型和预存耐药变异的研究  

Research of hepatitis B virus genotyping and pre-existing antiviral resistance mutation in Shenzhen area

作　　者：陈灿峰[1] 徐传彬[1] 黄雪辉[1] 

机构地区：[1]深圳市西乡人民医院,广东深圳518102

出　　处：《国际检验医学杂志》2014年第23期3190-3191,共2页International Journal of Laboratory Medicine

摘　　要：目的了解深圳地区临床慢性HBV感染患者的HBV基因型和预存耐药变异情况。方法应用PCR结合反向点杂交法检测244例临床慢性HBV感染血清样本的基因型和预存耐药变异情况。结果 244例慢性HBV感染症状的患者中,共发现有3种HBV基因型,其中B型143例,占58.6%;C型100例,占41%;D型1例,占0.4%;244例慢性HBV感染症状的患者中,有38例检出HBV预存耐药变异,预存耐药变异的总检出率为15.57%,其中与拉米夫定耐药相关的为22例(9.01%),与阿德福韦酯耐药相关的为16例(6.56%)。结论深圳地区HBV基因型以B、C型为主,慢性HBV感染者其预存耐药率较高。对慢性HBV感染者进行HBV基因分型及预存耐药检测,对预测疾病进展及指导个体化治疗具有重要意义。Objective To understand the hepatitis B virus（HBV）genotyping and pre-existing antiviral resistance mutation in the patients with clinical chronic HBV infection in Shenzhen area.Methods Serum samples in 244 cases of chronic HBV infection were detected for HBV genotyping and pre-existing antiviral resistance mutation by using PCR combined with reverse dot hybridization. Results In 244 cases of chronic HBV infection,3 kinds of genotype were found,including 143 cases（58.6%）of genotype B,100 ca-ses（41%）of genotype C and 1 case（0.4%）of genotype D;38 cases（15.57%）of pre-existing antiviral resistance mutation were de-tected out.The total detection rate of pre-existing antiviral resistance mutation was 15.57%,in which 22 cases（9.01%）were related with lamivudine resistance and 16 cases（6.56%）were related with adefovir dipivoxil resistance.Conclusion The genotype B and C are the main genotypes in Shenzhen area,and the incidence rate of pre-existing antiviral resistance mutation in the patients with chronic HBV infection is relatively high.The detection of genotyping and pre-existing antiviral resistance mutation of HBV has the important significance to predict the disease progression and guide the individulized treatment.

关 键 词：乙型肝炎病毒 基因型 预存耐药变异 

分 类 号：R512.62[医药卫生—内科学]