探讨尿沉渣分析仪、尿干化学分析仪及光学显微镜3种方法检测尿液红细胞的一致性  

Study on consistency of urinary sediment analyzer,urine dry chemistry analyzer and optical microscope in detecting urine erythrocyte

作　　者：肖凤静[1] 郑善銮[1] 何娟[1] 卢佩[1] 林婕[1] 郝晓柯[1] 

机构地区：[1]第四军医大学西京医院检验科,陕西西安710032

出　　处：《国际检验医学杂志》2014年第23期3241-3243,共3页International Journal of Laboratory Medicine

摘　　要：目的比较并分析SYSMEX UF1000i全自动尿沉渣分析仪、Arkray AX-4030尿干化学分析仪及光学显微镜检测尿液红细胞的一致性。方法随机抽取427例患者新鲜尿液标本,分别用SYSMEX UF1000i全自动尿沉渣分析仪、Arkray AX-4030尿干化学分析仪及OLUMPUS光学显微镜进行检测,比较分析3种检测方法检测尿液红细胞的结果是否一致。结果以显微镜检查作为对照,SYSMEX UF1000i全自动尿沉渣分析仪对红细胞检测的敏感度、特异性为82.84%和86.35%,Arkray AX-4030尿干化学分析仪对红细胞检测的敏感度、特异性分别为89.55%和83.96%,SYSMEX UF1000i全自动尿沉渣分析仪对红细胞检测结果的Kappa值为0.580,一致性高;Arkray AX-4030尿干化学分析仪对红细胞检测结果的Kappa值为0.625,一致性高,SYSMEX UF1000i全自动尿沉渣分析仪与Arkray AX-4030尿干化学分析仪对红细胞检测结果的Kappa值为0.324,一致性较弱。结论将SYSMEX UF1000i全自动尿沉渣分析仪、Arkray AX-4030尿干化学分析仪作为过筛试验需结合光学显微镜检查进行复检,才能快速、准确地为临床提供有效、可靠的检验结果。Objective To analyze the consistency of the SYSMEX UF1000i automatic urinary sediment analyzer,Arkray AX-4030 urine dry chemistry analyzer and optical microscope in detecting urine erythrocyte.Methods The fresh urine specimens from 427 patients were randomly extracted and tested by the SYSMEX UF1000i automatic urinary sediment analyzer,urine dry chemistry analyzer and OLUMPUS Arkray AX-4030 optical microscope.Then the consistency of the results for detecting urine erythrocyte was compared among three kinds of detection method.Results With the microscopic examination as control,the sensitivity and spe-cificity of the SYSMEX UF1000i automatic urinary sediment analyzer for detecting urine erythrocyte were 82.84% and 86.35% re-spectively,which of the Arkray AX-4030 urine dry chemistry analyzer were 89.55% and 83.96% respectively.There was a high consistency between the SYSMEX UF1000i automatic urinary sediment analyzer and the optical microscope for detecting urine e-rythrocyte and the Kappa value was 0.580.There was also a high consistency between the Arkray AX-4030 urine dry chemistry analyzer and the optical microscope for detecting urine erythrocyte and the Kappa value was 0.625,while the consistency between the SYSMEX UF1000i automatic urinary sediment analyzer and the Arkray AX-4030 urine dry chemistry analyzer was weaker and the Kappa value was 0.324.Conclusion With the detection by the SYSMEX UF1000i automatic urinary sediment analyzer and the Arkray AX-4030 urine dry chemistry analyzer as a screening test,it should need to combine with the optical microscopy to conduct recheck for providing the effective and reliable test results quickly and accurately.

关 键 词：尿液分析 尿红细胞 尿沉渣 尿干化学分析仪 

分 类 号：R446.12[医药卫生—诊断学]