激活视网膜水平细胞NMDA受体对GABA转运体的抑制  

Inhibition of GABA Transporters by NMDA Receptors on Retinal Horizontal Cell

作　　者：姜晓东[1] 周桂凤[1] 汪耀珠[1] 

机构地区：[1]湖南师范大学医学院,长沙410013

出　　处：《重庆科技学院学报（自然科学版）》2014年第6期160-164,共5页Journal of Chongqing University of Science and Technology：Natural Sciences Edition

基　　金：湖南省教育厅资助科研项目(12B074);湖南省卫生厅资助科研项目(B2012-062)

摘　　要：目的:在视网膜水平细胞上研究激活NMDA受体对GABA转运体的调节作用。方法:采用膜片钳全细胞记录,分析水平细胞NMDA受体在激活前后的GABA转运体电流变化,并分析Ca2+在其中的作用。结果:当灌流0.1mmol/L的NMDA 30s后,1 mmol/L GABA所引发的GABA转运体电流下降到了对照水平的(78.01±3.10)%(n=5);当细胞外Ringer’s液的Ca2+被去除后,NMDA的抑制效应消失;当胞内Ca2+库的Ca2+释放和重吸收被阻断,或者胞内Ca2+被BAPTA缓冲后,NMDA的抑制效应减弱。结论:激活视网膜水平细胞的NMDA受体会抑制GABA转运体电流,细胞内Ca2+参与调节过程。Objective： The modulation of NMDA receptors on GABA transporters was investigated on retinal horizontal cell. Methods： Changes of GABA transporter current were observed after activation of NMDA receptors via whole cell recording,and intracellular Ca^2 ＋ was also studied. Results： GABA transporter currents（ by 1 mmol / L GABA） were decreased to 78.01% ± 3.10%（ n = 5） of control level after application of 0.1 m M NMDA. The inhibition of GABA transporters was attenuated by blockage of Ca^2 ＋ release and uptake of intracellular Ca^2 ＋ store. The effect was also decreased when BAPTA was added to intracellular solution. Conclusion： inhibition of GABA transporters can be modulated by NMDA receptors on retinal horizontal cell,and intracellular Ca^2 ＋ plays an important role.

关 键 词：视网膜 NMDA受体 GABA转运体 CA^2+ 

分 类 号：Q436[生物学—生理学]