胡芦巴子叶中油脂的制备工艺及GC-MS分析  

Preparation Technology of Lipid from Fenugreek Fat and GC-MS Analysis

作　　者：马建龙[1,2] 薛妍[1] 马志宁[1] 吴晶晶[1] 晋晓勇[1] 

机构地区：[1]宁夏大学化学化工学院,宁夏银川750021 [2]宁夏大学天然药物工程技术研究中心,宁夏银川750021

出　　处：《宁夏大学学报（自然科学版）》2014年第4期352-357,共6页Journal of Ningxia University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(21365016);企业横向合作基金资助项目(414-0091)

摘　　要：研究胡芦巴油脂的提取工艺及脂肪酸的组成成分.以胡芦巴子叶为原料,采用超临界 CO2萃取法提取,经皂化、甲酯化后,用 GC-MS 进行分析测定.结果表明,当粉碎度为60-80目,提取时间为3 h,提取釜压力为30 MPa,CO2的体积流量为20 L/h时,胡芦巴子叶中油脂的提取率为10.30%.面积归一法计算表明,脂肪酸组成以亚麻酸、亚油酸、硬脂酸及棕榈酸为主,占油脂总量的92.56%,其中亚麻酸等不饱和脂肪酸占总量的55.43%,香味成分γ-十一内酯及γ-十二内酯的质量分数为3.89%.对于胡芦巴油脂的综合利用开发提供了有价值的参考.This article is about the study of fenugreek grease’s extracting process and the composition of aliphatic acid.Taking fenugreek fat as the material,the authors extract with super-critical CO2 extraction method and make assay determination with GC-MS after saponification and methyl esterification.The result shows that the fat extraction efficiency of fenugreek cotyledons reaches 10.30% when comminution degree turns to 60-80 mesh,extraction period 3 h,extraction of reactor pressure 30 MPa,and volume flow rate of CO2 20 L/h. The calculation from area normalization method shows that the major compositions of fatty acid are linolenic acid,linoleic acid,stearic acid,and palmitic acid,accounting for 92.56% of overall grease,of which the unsaturated fattyacid,like linolenic acid,55.43%,the mass fraction of either fragrance component-γ-Undecalactone and γ-Dodecalactone is 3.89%. The article provides valuable reference for the comprehensive utilization and exploitation of fenugreek cotyledons.

关 键 词：胡芦巴 超临界CO2萃取法 油脂 

分 类 号：O657[理学—分析化学]