补骨脂酚对人肾上腺皮质癌H295R细胞中睾酮和雌二醇分泌的影响  

Effects of bakuchiol on secretion of testosterone and estradiol from H295R cells in human with carcinoma of adrenal cortex

作　　者：毛浩萍[1] 牛子长[2] 王兴业[1] 邱燕荣 

机构地区：[1]天津中医药大学中医药研究院,天津300193 [2]天津中医药大学第一附属医院,天津300193

出　　处：《天津中医药大学学报》2014年第6期347-350,共4页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81303247);教育部高等学校博士学科点专项科研基金项目(20121210120009)

摘　　要：[目的]研究补骨脂酚对人肾上腺皮质细胞(H295R)睾酮及雌二醇分泌的影响。[方法]实验分为空白对照组及补骨脂酚不同浓度组,分别使用CCK-8法及酶联免疫吸附法(ELISA法)检测补骨脂酚对H295R细胞活力、睾酮和雌二醇分泌的影响;同时采用实时荧光定量聚合酶链式反应法(Real-time PCR)检测补骨脂酚对睾酮和雌二醇合成酶CYP17A1和CYP19A1 m RNA表达的影响。[结果]补骨脂酚对睾酮合成酶CYP17A1 m RNA无显著影响,但能上调雌二醇合成酶CYP19A1 m RNA含量,具有减少睾酮分泌增加雌二醇分泌的作用。[结论]补骨脂酚促进H295R细胞中雌激素的产生。[Objective] To study the effects of bakuchiol on testosterone and estradiol secretion from H295 R cells. [Methods] The researches were divided into control group and bakuchiol groups. The effects of bakuchiol on cellular activites and the secretion of testosterone and estradiol were detected by CCK-8 method and ELISA method.The levels of CYP17A1 and CYP19A1 m RNA were also detected by Real-time PCR.[Results] Bakuchiol could not affect CYP17A1 m RNA leverl but could up-regulate CYP19A1 m RNA. Bakuchiol had the effect of decreasing the secretion of testosterone but increasing the secretion of estradiol. [Conclusion] Bakuchiol can stimulate estradiol secretion from H295 R cells.

关 键 词：补骨脂酚 肾上腺皮质细胞 睾酮 雌二醇 

分 类 号：R285.5[医药卫生—中药学]