高效液相色谱法测定菟丝子中绿原酸、对-香豆酸和金丝桃苷的含量  

Determination of chlorogenic acid,p-coumarin and hyperin of Cuscuta chinensis Lam. by high performance liguid chromatography

作　　者：田雨霏 刘姣[1] 李晋[1] 马琳[1] 何俊[1] 刘二伟[1] 常艳旭[1] 

机构地区：[1]天津中医药大学天津市现代中药重点实验室,天津300193

出　　处：《天津中医药大学学报》2014年第6期372-374,共3页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：高等学校博士学科点专项科研基金(20101210120007);国家自然科学基金(81001632);中国博士后基金(20100480657);教育部科学技术重点项目(210013);天津市科技支撑计划重大项目(10ZCZDSY12400)

摘　　要：[目的]建立同时测定菟丝子中绿原酸、对-香豆素和金丝桃苷的含量的高效液相色谱法,为菟丝子的质量标准建立提供依据。[方法]色谱柱为依利特ODS C18（4.6 mm×250 mm,5μm）;流动相为甲醇-0.05%甲酸水溶液,梯度洗脱,流速为1 m L/min,检测波长为360 nm。[结果]在此色谱条件下,3种成分可完全分离。绿原酸、对-香豆素和金丝桃苷的线性分别为2.00-100μg/m L（r=0.999 3）,6.25~100μg/m L（r=0.999 1）,0.50~50μg/m L（r=0.999 1）。平均回收率分别为100%（RSD=3.2%）、102%（RSD=1.7%）和102%（RSD=3.1%）。[结论]该方法简便可行,重复性好,可用于菟丝子的质量评价。[Objective] To establish a sensitive method for simultaneous determination of chlorogenic acid, pcoumarin and hyperin in order to provide some evidences for the quality control of Cuscuta chinensis Lam..[Methods] Methanol and 0.05% formic acid were used as mobile phase. The sample was separated on an ODS C1825 column（4.6 mm×250 mm,5 μm） by gradient elution. The flow rate was 1.0 m L/min. The wavelength was set at360 nm. [Results] Chlorogenic acid, p-coumarin and hyperin were well separated by HPLC. Linearity ranges were2.00~100 μg/m L（r=0.999 3）, 6.25~100 μg/m L（r=0.999 1） and 0.50~5 0 μg/m L（r=0.999 1） for chlorogenic acid, pcoumarin and hyperin, respectively.[Conclusion] The simple and feasible HPLC method was established to evaluate the quality of Cuscuta chinensis Lam..

关 键 词：菟丝子 高效液相色谱法 绿原酸 对-香豆素 金丝桃苷 

分 类 号：R285.5[医药卫生—中药学]