番茄micro-TOM活体再生体系的建立  

Establishment of Tomato micro-TOM Regeneration System in Vivo

作　　者：曹慧颖[1] 王玉莹[1] 孙建坤[1] 刘玲莉[1] 张立军[1] 阮燕晔[1] 夏润玺[1] 

机构地区：[1]沈阳农业大学生物科学技术学院、辽宁省植物基因工程技术研究中心,辽宁沈阳110866

出　　处：《中国蔬菜》2014年第12期27-29,共3页China Vegetables

基　　金：国家自然科学基金项目(31300164);教育部博士学科点专项科研基金(20122103120004);沈阳农业大学大学生科技创新资助项目

摘　　要：番茄micro-TOM是生物学研究的模式植物,它的离体再生体系对遗传转化及发育生物学研究至关重要。但是microTOM组培再生体系费时费力,容易污染。本试验通过对活体植株进行去头去腋芽诱导,建立了高效快捷、不依赖外源激素的番茄micro-TOM活体再生体系,为简化番茄遗传转化奠定了基础,也为植物再生机理研究提供了良好的实验材料。Tomato （Solanum lycopersicum L.）micro-TOM is a model organism for biology study. Its regeneration system in vitro is of vital importance for genetic transformation and developmental biology study. But tomato micro-TOM tissue culture is not only time-consuming and tedious, but also frequently polluted by microorganism. This experiment introduces a new method of regeneration system in vivo by removing axillary and apical buds of tomato. The method has high speed and efficiency, and does not rely on exogenous hormone. It has layed a foundation for simplifying tomato genetic transformation and provided a good material for plant regeneration mechanism study.

关 键 词：植物再生 组织培养 再生机理 遗传转化 番茄micro-TOM 

分 类 号：S641.2[农业科学—蔬菜学]