杨树生物能源真菌的筛选研究  

Screening Bio-Energy Fungi from Populus

作　　者：赵桂华[1,2] 蒋继宏[2] 赵楠[1] 

机构地区：[1]江苏师范大学江苏省药用植物生物技术重点实验室,江苏徐州221000 [2]江苏农林职业技术学院,江苏句容212400

出　　处：《西南林业大学学报（自然科学）》2014年第6期1-8,共8页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家自然科学基金项目(31170605)资助

摘　　要：为了筛选杨树上潜在的生物能源油脂真菌,对5种杨树的健康树皮、韧皮部、边材和叶片真菌组织进行分离和鉴定,并检测了菌丝和孢子内含油脂。结果表明,试验共分离4 674块组织,得到真菌菌落4 501个,占分离组织总数的96.3%,细菌菌落173个,占3.7%。其中非产油脂真菌菌落4 043个,占89.8%;产油脂菌落458个,占整个菌落总数的10.2%。根据真菌的形态特征进行鉴定,其隶属于接合菌门、担子菌门和半知菌亚门10属,80%为半知菌亚门的真菌,其中,腐皮镰刀菌是一株高产油脂的真菌。产油脂真菌的出现与杨树种类和季节无相关性,而与真菌种类有关系。In order to screen some potential oleous fungi,fungus tissue of healthy bark,phloem,sapwood and leaf from five Populus were isolated,and detection mycelia and grease content in spore.The results showed that there were 4 501 fungus colonies from 4 674 poplar tissues,accounting for about 96.3%,and bacteria col-onies accounting for 3 .7%.Among of them,no-oleous fungal colonies were 4 043 ,accounting for 89 .8%,and oleous fungal colonies were 45 8 ,accounting for 1 0 .2%.The authentication results of oleous fungals by morpho-logical characteristics showed that,these oleous fungals belonged to Zygomycota,Basidiomycota and Deuteromy-cotina,and 80% of them belonged to imperfect fungi.Among them,Fusarium solani was a fungus with high oil yield.No correlation between the fat producing fungi appeared and Populus species and seasonal,but correlated to the fungal species.

关 键 词：杨树 生物能源 油脂真菌 筛选 鉴定 

分 类 号：S718.81[农业科学—林学]