三七根腐病株根际土壤真菌群落组成与碳源利用特征研究  

Characterization of fungal community composition and carbon source utili‐zation in the rhizosphere soil of Panax notoginseng suffering from root‐rot disease

作　　者：吴照祥[1] 郝志鹏[1] 陈永亮[1] 曾燕[2] 郭兰萍[3] 黄璐琦[3] 王勇[4] 陈保冬[1] 

机构地区：[1]中国科学院生态环境研究中心城市与区域生态国家重点实验室,北京100085 [2]中国药材公司,北京100097 [3]道地药材国家重点实验培训基地中国中医科学院,北京100700 [4]文山学院文山三七研究院,云南文山663000

出　　处：《菌物学报》2015年第1期65-74,共10页Mycosystema

基　　金：国家自然科学基金(41101245);国家科技支撑计划项目(2012BAI29B02)

摘　　要：根腐病是药用植物三七Panax notoginseng的多发性病害,而土壤真菌群落组成与根腐病的发生存在一定的联系。利用末端限制性酶切片段长度多态性分析和Biolog技术研究了云南文山三七种植园健康和发病三七植株根际土壤真菌群落结构和碳源利用特征,结果表明健康和发病植株的根际土壤真菌多样性和代谢活性差异不显著,但是真菌群落组成存在差异。健康和发病植株根际土壤都以小茎点霉Phoma exigua及镰孢菌属Fusarium真菌为主要种群,而健康植株根际土壤中火丝菌属Pyronema和被孢霉属Mortierella等真菌类群的相对丰度显著高于发病植株根际土壤,尖孢镰孢菌F.ox‐ysporum和刺座霉属的Volutella colletotrichoides的相对丰度则显著低于发病植株。Root‐rot is a common disease in plantation of Panax notoginseng, which is more or less affected by community struc‐ture and function of rhizosphere soil fungi. In the present study, rhizosphere soil samples were collected in healthy and diseased fields of P. notoginseng in Wenshan of Yunnan Province for analysis of community composition of soil fungi by terminal re‐striction fragment length polymorphism（T‐RFLP） and utilization of carbon sources by Biolog FF microplate. The results indicated that the diversity and metabolic activity of rhizosphere soil fungi had no significant difference between the rhizosphere soil of healthy and diseased plants, but their fungal community compositions were different. The most abundant fungi in rhizosphere soil of both healthy and diseased plants included Phoma exigua and Fusarium spp. However, the isolation rates of Pyronema spp. and Mortierella spp. were much higher in the rhizosphere soil of healthy plants than that of diseased plants. On the contrary, the isolation rates of F. oxysporum and Volutella colletotrichoides in the rhizosphere soil of healthy plants were obviously lower than in that of diseased plants.

关 键 词：三七 根腐病 真菌群落 末端限制性酶切片段长度多态性分析(T‐RFLP) Biolog技术 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]